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Ji Cheng Xi Lin S.E.Scheuermann and Rong Yi  
Using Lichro CART Superspher CH-8,4um,250×4mm i.d.as stable phase and acetonitrile-Na-Acetat as mobile phase,the amino acids in the standard solution and in the hydrolyzed feedstuff solutions were derivatized with 9-fluorenylmethyl chloroformate(FMOC) at room temperature,separated with the reversed-phase system by adjusting the elution gradient and polarity of the mobile phase,and finally determined by fluoresence detector at emmition 310 nm and excitation 260 am.The results showed that FMOC had high sensitivi- ty and stability.A linearity was obtained between the concentration of amino acid and intensity of fluoresence within the range of concentrations in our experiment.The CV%of amino acids determined from the standard solutions was less than 4%,with the exception of lysine(CV=9.6%,n=12).The CV of the different hydrolyzed feedstuff solutions was less than 10%.
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Chinese Journal Full-text Database 3 Hits
1 Ji Cheng Xu Wangen Rong Yi (Beijing Agricultural University) Xie Zhihao Wang Yushuen (Beijing Animal Husbandry Bureau);STUDIES OF THE CORRELATION OF THE LYSINE AVAILABILITIES OF POULTRY FEEDS BY DBL AND SIBBALD"TME"METHODS USING CAECETOMIZED COCKERRELS[J];;1990-03
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1 An Yongyi Zhou Yuping Guo Yuming Ding Jiaoli Zhu Yuqing(College of Animal Science and Technology,China Agricultural University,Beijing,100094);STUDIES ON DEVELOPMENT OF DIGESTIVE ENZYMES IN BROILER CHICKS[J];ACTA ZOONUTRIMENTA SINICA;1999-01
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【Secondary References】
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3 Wang Weibo,Zhao Guoqi,Han Qingguang;Study on the optimum condition of soybean digested by pepsin-trypsin in vitro[J];China Feed;2009-02
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