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Construction of AFLP Analysis System in Apple

Zhu Jun Wang Tao Li Guangchen Zhao Yujun Zhang Wen Zhou Aiqin(College of Horticulture,CAU)  
AFLP(Amplified Fragment Length Polymorphism)analysis system was established with 12 apple rootstock genotypes( Malus ). The results of several reactions during AFLP analysis have been detected such as DNA extraction,double enzymes restriction,adapter ligation,pre amplification,labeling and selective amplification. The AFLP fingerprintings of 12 apple rootstock genotypes with primer P32M64 have been performed,which had 105 clear and strong amplification bands with bright background. The factors which affect AFLP analysis were discussed.
【Fund】: 国家自然科学基金 !39770 52 3;; 教育部博士点基金资助项目
【CateGory Index】: S661.1
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