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《Journal of China Agricultural University》 2002-05
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Establishment of E-Genome-Specific RAPD and SCAR Markers for Thinopyrum spp.

You Mingshan Li Baoyun Tang Zhaohui Liu Shoubin Song Jianmin Mao Shanfeng Liu Guangtian (College of Crop Science, China Agricultural University, Beijing 100094,China)  
A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516) in Th. bessarabicum. Based on the sequence of OPF03 1291 , two pairs SCAR primers were designed. With OPF03 and primers P3 and P4, RAPD and SCAR analyses were performed on T.aestivum, T.aestivum Th.intermedium partial amphidiploid, Th.elongatum, Th.intermedium, wheat Th.elongatum substitution and addition lines. The results of PCR amplification showed that the RAPD marker was not appeared in materials with genome E e, whereas SCAR marker was appeared in all E genome possessed materials. The SCAR marker derived from the E b genome specific RAPD fragment could be used in the meantime to detect the chromosome of E e genome under common wheat background.
【Fund】: 国家自然科学基金重点资助项目 (39930 110 );; 北京市自然科学基金重大资助项目 (6 990 0 0 1)
【CateGory Index】: S512.1
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