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《Journal of China Agricultural University》 2003-06
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Purification of XynB from Streptomyces cirratus

Ding Changhe, Jiang Zhengqiang, Li Lite, Li Xiuting, Zhang Yan (College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China)  
To study further the property of xylanases from Streptomyces, purification of a xylanase from Streptomyces cirratus D-10 was investigated. A xylanase, named XynB, was purified by stepwise ammonium sulphate precipitation (20%-50% saturation) and Q-sephrose FF ion exchange chromatography. After two-step treatment, ten-fold purification was obtained with 24.0% activity recovery. The purified XynB showed as a single protein band on SDS-PAGE that molecular weight of the enzyme was estimated to be 22.0 ku by SDS-PAGE, and belonged to one of lower molecular weight xylanases.
【Fund】: 国家“十五”攻关课题 (2 0 0 1BA70 8B0 4 0 6 ) ;; 教育部研究重点项目
【CateGory Index】: Q936
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【Co-citations】
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1 CHEN Lei;ZHAO Xiangying;LIU Jianjun;College of Bioengineering, Qilu University of Technology;Food & Fermentation Engineering Key Lab of Shandong Province;;Purification and enzymatic properties of xylanase from Paenibacillus campinasensis[J];中国酿造;2016-07
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【Co-references】
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