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《Journal of China Agricultural University》 2007-01
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Photoprotective effect of melanin derived from White Silky Fowl on Drosophila melanogaster against ultraviolet radiation

Wang Zhepeng(1,2),Deng Xuemei1,Wang Anru3(1.College of Animal Science and Technology,China Agricultural University,Beijing 100094,China;2.Vocational and Technical College of Inner Mongolia Agricultural University,Baotou 014109,China;3.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China)  
The Yellow strain of Drosophila was used to evaluate photoprotective power of melanin against ultraviolet radiation(UVR).Flies were firstly exposed to UVR for 2 or 5 min,and then reared in culture media with melanin at concentrations of 0.02 or 0.2 mg/mL for a week.Flies were then transferred to basic culture media to determine their fecundity and SOD activity.The lower and upper quartile and mean life spans which were(14.5±1.3),(23.5±2.1) and(15.7±7.1) days respecytively,of y in 5 min UV+0.2 mg/mL melanin group were significantly(P0.05) higher than those((8.0±1.4),(16.0±3.3) and(13.5±6.4) days) in 5 min UV group.Fecundity(mean number of offsprings per couple) of y in 2 min UV+0.2 mg/mL melanin group was(50.0±11.4) or 156.4%(P0.05) more than that of y(19.5±12.5) in 2 min UV group.We also found that melanin may play a role in protecting SOD in flies exposed to UVR from degrading.Although the SOD activities((209.1±13.1) and(213.25±7.7) μmol/(mg·min)) of flies in 5 min UV+0.02 mg/mL melanin and 5 min UV+0.2 mg/mL melanin groups were higher than those((204.2±11.5) μmol/(mg·min)) of flies in 5 min UV group,the differences were insignificant.Therefore melanin can extend life span,improve fecundity and increase SOD activity of y exposed to UVR through scavenging free radicals in y.At the same time the positive role of melanin in fecundity may be attributed to its protection of germ cells.
【Fund】: 国家自然科学基金资助项目(30471233)
【CateGory Index】: R285
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