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Estimation of protein intestinal digestibility of ruminant feedstuffs with mobile nylon bag technique and Three-step in vitro procedure

Yue Qun~1,Yang Hongjian~1,Xie Chunyuan~1,Yao Xuebo~1,Wang Jiaqi~2(1.College of Biological Sciences,China Agricultural University,Beijing 100094,China;2.State key Laboratory of Animal Nutrition/Institute of Animal Science,China Academy of Agricultural Sciences,Beijing 100094,China)  
Apparent small intestinal digestibility(Idg) of 15 feedstuffs was determined by the mobile nylon bag technique(MNBT) and three-step in vitro procedure respectively.Residues collected after 16?h rumen incubation by in situ technique were applied for the MNBT procedure through a T-type proximal duodenal cannula and in vitro procedure.The Idg determined by MNBT were 90.60%(corn gluten meal),82.31%(rape seed meal),96.32%(soybean meal),80.62%(cotton seed meal),95.58%(peanut cake),67.89%(wheat bran),89.86%(maize),74.33%(brewer's grains),58.65%(rice bran),79.79%(corn germ meal),90.42% (distillers dried grains with soluble),40.00%(alfalfa hay),44.88%(chinese wildrye),35.52%(corn straw) and 10.52%(corn silage),respectively.The Idg of rumen undegraded crude protein(RUP) between the MNBT and three-step in vitro procedure was linearly correlated.The apparent small intestinal digestibility differed among feedstuffs(10%~96%).A linear regression equation was presented between the MNBT and three-step in vitro procedure(Y=1.1851X5.9468,n=15,R~2=0.8912,where Y represents apparent Idg of CP with the mobile nylon bag technique,and X represents the digestibility estimated by three-step in vitro procedure),which might be a simple,cheap,convenient and reliable method for feed evaluation in ruminants.
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