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《Journal of Agricultural Biotechnology》 1996-01
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Construction of Plastid Transformation Vectors of Bt Toxin Genes and Their Insecticidal Toxicity Test

Ren Yanguo Zhang Zhonglin Zou Zhurong Yu Xiaolan Ni Peichong Shen Guifang (Bioteehnology Resaerch Center,CAAS,Beijing 100081)  
Plastid transformation vectors pTRS1,pTRS2 and pTRS3 were constructed by inserting 3.5 kb full-length Bt toxin gene and its 2.1 kb、1.8 kb 3' truncated forms into the plasmid containing plastid genome homologous fragment RTP and TO respectively.Insecticidal toxicity assay of the rude pro- teins of their host E.coli strains showed that all of them had 100% mortality to two instar of Heliothis armigera and high toxicity to its three instar represented by pTRS1 with the mortality up to 95%.There- by,all of above vectors can be used for plastid transformation to obtain Bt toxin gene transgenic plants.
【Fund】: 国家自然科学基金项目资助
【CateGory Index】: S476
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