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《JOURNAL OF AGRICULTURAL BIOTECHNOLOGY》 1999-01
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Identification and Screen of Waxy Protein in Wheat

Yao Danian; Wang Xinwang; Liu Zhiyong; Liu Guang tian (College of Plant Sci & Tech, China Agricultural University, Beijing 100094 )  
The types of sub-units of waxy protein in wheat including Chinese Spring (CS), nulli-tetrasomic lines of CS, Chinese Local variety-Baihuo and more than 400 wheat Varieties or lines collected from various countries were identified,43 varieties (or lines ) were the types with one or two sub-units of waxy protein, 3 varieties were the types with null Wx-D1 subunits of waxy protein. The types of subunits of waxy protein of einkorn, emmer and Tibeten wheat were analyzed, and a translocation between 4AL and 7BS chromosome might occur in process of evolution from diploid to hexaploid wheat were verified. The fragment with Wx-B1 gene encoded Wx-B1 protein of 7BS, was translated to 4AL. PCR methods were used for identifying the Wx genes as well as the inheritance of waxy protein were studied.
【Fund】: 国家自然科学基金!39770460;;安徽省自然科学基金
【CateGory Index】: S512.103.7
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