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《JOURNAL OF AGRICULTURAL BIOTECHNOLOGY》 1999-02
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Study on the Conversion of RFLP Markers co-segregated with Pm4a to Sequenced-tagged-site Markers

Liu Jinyuan; Liu Dajun; Tao wenjing; Li Wanlong; Chen Peidu(Key Open lab of Agri. Ministry for Crop Cytogenetics, Naming Agricultural University, Nanjing 210095)  
Based on the sequence data ofclone BCD 1231, a pair of 23-hp primers were designed. When the annealing temperature is 45~50℃, a specific band of about 1 .7 kb was amplified from samples containing Pace4a gene. Further amplification using the primers found the specific band was only present in the tasted cultivars and lines containing Pm4a and Pm4b genes, but absent in the gCnotypes without Pm4 gene. As PCR-based markers may be of greater interest of breeders, further studies on PCRbased markers are proceeding.
【Fund】: 国家自然科学基金!39770413;;国家‘863’计划!Z-17-04-01
【CateGory Index】: S435.121.46
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