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《JOURNAL OF AGRICULTURAL BIOTECHNOLOGY》 2000-01
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RAPD and ISSR Analysis between Photoperiod Sensitive Genie Male Sterile Rice Nongken 58s and its Original Variety Nongken 58

Jiang Shuye1,2; Chen Qifeng2; Fang Xuanjun1 (1 Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, 2 Institute of Genetics and Crop Breeding, Fujian Agricultural University. Fuzhou 350002; (Corresponding author  
RAPD and ISSR polymorphisms between photoperiod sensitive genie male sterile rice Nongken 58s and its original variety Nongken 58 were analyzed with their genomic DNAs as PCR template. The results are as following: 154 RAPD and 78 ISSR primers generated amplified products in RAPD and ISSR analyses. Among them, 8 RAPD and 7 ISSR primers produced polymorphic bands. Among the polymorphic bands by RAPD-PCR. some of them are particular with Nongken 58s, some of them are connected with Nongken 58, and some of them appeared both in Nongken 58s and in Nongken 58. However. ISSR polymorphic bands appeared only in either Nongken58 or Nongken58s. There are 23 specific RAPD bands and 13 ISSR specific bands in total, among which 10 specific RAPD bands and 7 specific ISSR bands only appeared in Nongken58s. Undenatured polyacrylamide gel electrophoresis and rapid silver staining were employed to isolate and show RAPD and ISSR products. This method is considered suitable for being popu larized for that it is convent ent, quick and it dramatically promotes the resolution compared with agarose gel electrophoresis and EB staining. Some other relative problems are also discussed in this paper. and we think that polymorphic DNA fragments can be identified by BSA analysis or by Northern blotting with sterile and fertile mRNA produced during the transformation of pollen fertility.
【Key Words】： photoperiod sensitive genie male sterile rice RAPD ISSR
【Fund】： 国家863计划!Z16－02－03;; 福建省自然科学基金!C97027
【CateGory Index】： S188
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