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《Journal of Agricultural Biotechnology》 2001-01
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mRNA Differential Expression Analysis Between Fertile and Sterile Young Panicle of Photoperiod Sensitive Genic Male Sterile Indica Rice

Jiang Shuye1,3 Lu Bei2 Chen Qifeng3 Fang Xuanjun1,2 1 Hainan Provincial Institute of Tropical Agricultural Resources, .Sanya 572000; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081; 3 Institute of Genetics and  
The mRNAs from both fertile and sterile young panicle of photoperiod sensitive genie male sterile indica rice Hs-1 are analyzed by DD-PCR. The results are as following: Among 148 DD-PCR reactions, 10 reactions produce repeatable differentially exptessed bands. In those 10 reactions, 4 of them each show 1~4 differential bands that are found either only in fertile young panicle or in both fertile and sterile young panicles. Each of the rest 6 reactions amplified more than 4 differential bands from both fertile and sterile young panicles. There are 43 specific bands in total, among which 21 specific bands may be related to sterility. We suggest the key to isolate sterile genes is to anchor the start period of differential expression of these genes during fertility transformation. It is considered that expression of sterile genes may also result in transcription of other mRNAs and thus produce additional differential bands. It is important to further investigate the relationship between the differential bands and sterility.
【Fund】: 国家863计划资助,项目编号: Z16-02-03
【CateGory Index】: S511.2
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