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《Journal of Agricultural Biotechnology》 2001-02
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Analysis On the Partial Sequence of Bovine Lactoferrin Gene by PCR-SSCP

Li Guohua; Zhang Yuan; Li Ning ( College of Animal Science and Technology, China Agricultural University; The Nationa1 Laboratories for Agrobiotechnology China Agricultural University, Beijing l00094)  
To detect the DNA variation of bovine lactoferrin gene, exon 7, l2 and 5' -regulatory region have been studied by PCR- SSCP After sequencing a 227 bp fragment of 5' -regulatory region of two mutational homozygotes, a G →A and a T →G substitution were identified in the sites of -926 and -9l5 respectively Meanwhile, this region also shows single base C insertion at -839 site and T insertion at -8ll site. The gene frequency of A and B are 0.898 and 0. l02 respectively.
【CateGory Index】: S823.911
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