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《Journal of Agricultural Biotechnology》 2002-03
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Assessment of Genetic Diversity of Melon (Cucumis melo )Germplasm Based on RAPD and ISSR

Liu Wanbo1 Song Ming1 Liu Fuzhong2 Wang Huaisong2(1.Department of Horticulture, Southwest Agricultural University, Chongqing 400716, China; 2. Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China )  
Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular markers were used to detect the genetic diversity among 37 melon (Cucumis melo L.) germplasms. A total of 21 RAPD primers and 10 ISSR primers (ISSR-10 primer was evenly composed of ISSR-2 and ISSR-4) were identified with polymorphism among the entries. For RAPD markers, 106 polymorphic bands were produced, percentage of polymorphic bands (PPB ) was 58.62%, mean polymorphism information content (PIC ) was 0.47. For ISSR markers,73 polymorphic bands were generated, PPB was 65.51%, mean PIC was 0.53. UPGMA cluster analysis based on two molecular markers data divided these melons into two groups: wild melon group and cultivated melon group. The genetic distance of each wild melon germplasms was far. Cultivated melon group was classified into two sub-groups, which were thick-peel melon and thin-peel melon. They were consistent with the grouping based on taxology systematic botany. Genetic similarity matrices revealed that the estimates of correlation coefficients of RAPD and ISSR were significantly correlated. Based on the results, RAPD and ISSR could be applied to detect genetic diversity analysis among melon germplasm.
【Fund】: 教育部、人事部留学回国人员启动基金资助项目。
【CateGory Index】: S652
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