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《Journal of Agricultural Biotechnology》 2003-03
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Establishment of the Method for Identifying the Extracellular Fatty Acid Binding Protein Genotypes with Allele-specific-PCR

Zhao Chunjiang Li Ning* Deng Xuemei (State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China)  
The genotypes of chick (Gallus gallus ) extracellular fatty acid binding protein (EX-FABP) are closely related to chick abdominal fat percentage. In the study, the method for identifying the EX-FABP genotypes with allele-specific PCR (AS-PCR) technique was established and the strategy of identifying single nucleotide polymorphisms with AS-PCR was discussed. A typical AS-PCR system consists of two allele-specific primers and ane common primer. PCR products can be resulted when a sample's DNA is amplified by the common primer and allele-specific primer which matches with the sequence of sample's DNA. There are not PCR products if the allele-specific primer does not match the sequence of the samples' DNA. According to whether there is PCR products or not, the genotypes of samples can be identified. For establishing an AS-PCR, it is very important to design proper number and kinds of mismatched bases at the 3′ end of the allele-specific primers where the mutation occurs and different genotypes are generated. When one mismatched base in the primers can not lead to different PCR results which can identify genotypes, the second or even the third mismatched bases should be added to the allele-specific primers. The different kinds of mismatched bases have different effect on the results of AS-PCR. If the mismatched bases are A-C or T-G, they have little effect on the amplifying efficiency of AS-PCR. But when the mismatched bases are A-G or C-C or C-G, they have dramatically negative effect on it. A modified AS-PCR, allele-specific and length-deferent PCR (ASLD-PCR) were established and introduced. In an ASLD-PCR system, two pairs of primer sites, each of them consists of an allele specific primer and a common primer,were used to amplify and identify genotypes. The PCR products of the two pairs of primers were different in the length and the different length of PCR products standed for different kinds of genotypes. So ASLD-PCR was more convenient to identify genotypes than AS-PCR.
【CateGory Index】: S831
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2 YU De-bing1,ZHANG Wei-feng2,HE Zong-liang1,WANG Lin-yun1,HUANG Rui-hua1,DU Wen-xing1,LIU Hong-lin1(1.Coll of Anim Sci and Tech,Nanjing Agric Univ,Nanjing 210095,China;2.Coll of Agron,Hebei Univ of Engin,Handan 056038,China);Study on the method to investigate the mutation in IGF-2 gene intron 3 G3072A and the distribution of polymorphism in different pig breeds[J];Journal of Yangzhou University(Agricultural and Life Science Edition);2008-02
3 Shan Tizhong Wang Yizhen;Research advances in the fatty acid binding protein gene in livestocks and poultry[J];China Feed;2006-19
4 WANG Shuai 1,2,WANG Dong 1,DU Wei-Hua 1,HAO Hai-Sheng 1,ZHU Hua-Bin 1,WANG Zong-Li 2 1.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100094,China;2.The Grassland Research Institute,Chinese Academy of Agricultural Sciences,Hohhot 010010,China;Progress on complex vertebral malformation in Holstein cattle[J];Hereditas;2007-09
5 WANG Hong-Mei, LI Jian-Bin, HOU Ming-Hai, WANG Chang-Fa, LI Qiu-Ling, ZHONG Ji-Feng Dairy Cattle Center of Shandong Academy of Agricultural Sciences, Ji’nan 250100, China;The development and application of method for detecting bovine complex vertebral malformation[J];Hereditas;2008-09
Chinese Journal Full-text Database 10 Hits
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2 ZHANG Li Fang, YANG Xiao Su, XIAO Bo Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China;Study of Genes of Spinal Muscular Atrophy in Children[J];Chinese Journal of Contemporary Pediatrics;2001-01
3 Mei Shuqi Peng Xianwen Li Yong Cheng Ni Song Zhongxi Zhou Pinghua Song Guangfu (Institute of Animal and veterinary sciences , HAAS,Wuhan 430209,China; Daqiao Husbandy Co. Ltd. of Huangshi of Hubei Province, Hitangshi 435000,China);Genetic Variants at Heart Fatty Acid-binding Protein Loci in Hubei White Pigs[J];Journal of Huazhong Agricultural;2004-02
4 Lin Yulong , Hou Jinlin ,Wang Zhanhui et al . Nanfang Hospital , First Military Medical University, Guangzhou 510515;CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS WITH HOT SPOT MUTATIONS IN HBV SIGNAL PEPTIDE REGION[J];Medical Journal of Chinese People's Liberation Army;2001-07
5 TIAN Yongqiang, TIE Guodong, CAO Yujing, ZHAO Xingxu & DUAN Enkui1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China;2. College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China;IGF-Ⅱ and IGFBP-1 reversely regulate blastocyst implantation in mouse[J];科学通报(英文版);2002-21
6 ZHANG Wei San, DING Jing Juan,MEN Xiao Yan. Affiliated Hospital of Guiyang Medical College, Guiyang 550004,China;Establishment of a method for detection of lamivudine-resistant mutants in sequence of HBV polymerase gene[J];Modern Practical Medicine;2002-07
7 Wu Yanning,Liu Honglin,Chen Jie,Xu Yinxue and Jiang Zhihua (College of Animal Science and Technology,Nanjing Agric Univ,Nanjing 210095);Bi PASA technique and its application in the study on the polymorphism of RYR1 gene[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;2000-02
8 YANG Xiao-jing,CHEN Jie,XU Qing-fu,WEI Xi-hui,ZHAO Ru-qian~* (Key Laboratory of Animal Physiology and Biochemistry,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China);Developmental changes of Myostatin and Myogenin genes expression in longissimus dorsi muscle of Erhualian and Large White pigs[J];Journal of Nanjing Agricultural University;2006-03
9 Liu Gentao,Chen Weihua,Zheng Yuanlin,Chen Jie and Han Zhengkang(Key Lab of Animal Physiology and Biochemistry,Ministry of Agriculture,Nanjing Agric Univ,Nanjing 210095);Development of a radioimmunoassay for insulinlike growth factor1[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;1999-02
10 Jiang Yunliang; Li Ning; Wu Changxin(College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018; College of Biology, College of Animal Science and Technology, China Agricultural University, Beijing 100094);Studies on the Molecular Biology of Myogenesis (Review)[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1999-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 LI Xing-fang et al(Animal Embryo Engineering Center,Qingdao Agricultural Unversity,Qingdao,Shandong 266109);Sequencing and Analyzing the Exon 2 of Heart Fatty Acid-Binding Protein (H-FABP) Gene of Bohai Black Cattle[J];Journal of Anhui Agricultural Sciences;2009-10
2 LU Li-Ping, MA Hong-Wei, JIANG Jun, WAMG Tao, HU Bin. Department of Laboratory Medicine, Shengjing Hospital, China Medical University, Shenyang 110004, China;Application of real-time PCR analysis of the SMN_1 gene in the carrier testing of spinal muscular atrophy[J];Chinese Journal of Contemporary Pediatrics;2007-05
3 Li Xiaoqiao,Yao Fengxia,Su Liang,Han Juanjuan,Meng Yan,Wang Zheng,Peng Yuanyuan,Dian Yan,Zhou Qing,Huang Shangzhi.Department of Medical Genetics,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,School of Basic Medicine Peking Union Medical College,WHO Collaborating Centre for Community Control of Hereditary Diseases,Beijing 100005,China;A Simplified Approach for Detecting Homologous Deletion of SMN1 Genes in Spinal Muacular Atrophy[J];Journal of Medical Research;2009-05
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