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《Journal of Agricultural Biotechnology》 2008-06
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CRS-PCR Polymorphism of GHR Gene in Chinese Holstein Cows and Its Relationship with Milk Production Traits

WANG Li-juan1,2,LI Qiu-ling1,WANG Chang-fa1,WANG Hong-mei1,LI Jian-bin1, GAO Yun-dong1,HOU Ming-hai1,ZHONG Ji-feng1 (1. Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China; 2. College of Public Health,Shandong University,Jinan 250012,China)  
Single nucleotide polymorphisms (SNPs) of exon 8 of growth hormone receptor (GHR) gene were detected in Chinese Holstein cows by PCR-RFLP. The results showed that the exon 8 of GHR gene digested by Tas Ⅰ could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661 respectively. The frequencies of genotype AA, AT and TT were 0.458, 0.350 and 0.191, respectively. Sequencing revealed that one single nucleotide mutation T→A at 4 962 bp in genotype TT compared with genotype AA resulted in an amino acid change of phenylalanine (TTT)→tyrosine(TAT). The result of Chi Square test indicated that the frequencies of pattern types of GHR gene digested by Tas Ⅰ were not fit with Hardy-Weinberg equilibrium in this population (P 0.05). The least squares means of fat percentage in genotype TT were higher than that in genotype AA (P 0.05).
【Fund】: 国家高技术研究发展计划(863)(No.2007AA10Z169);; 山东省良种工程项目(No.2007LZ10-06);; 山东省农业科学院科研创新基金项目(No.2006YCX028和No.2007YCX019);; 山东省农业科学院青年科研基金项目(No.2006YQN038)资助
【CateGory Index】: S823
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