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《Journal of Agricultural Biotechnology》 2009-03
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Analysis of Genetic Polymorphisms in Glycosylation-dependent Cell-adhesion Molecule1 Gene (GlyCAM1) of Cattle

LI Jing1,2, WANG Hong-mei1, WANG Chang-fa1, LI Qiu-ling1, LIU Nan2, HOU Ming-hai1,LI Jian-bin1, ZHONG Ji-feng1** (1.Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan 250100,China; 2, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109,China)  
The genetic polymorphism of glycosylation-dependent cell-adhesion molecule1 gene (GlyCAM1) in three different cattle (China Holstein cattle, Luxi Yellow cattle and Bohai Black cattle) were studied by CRS-PCR, PCR-SSCP and DNA sequencing. Sequencing results showed that two polymorphic sites were identified at position2081 (A/C) and 2417 (C/T) of the GlyCAM1 gene exon 3 and intron 3, respectively. At the 2081 site and the 2417 site the allelic frequencies of A and B were 0.7525/0.2475, 0.6112/0.3888, 0.3375/0.6625, 0.9046/0.0954, 0.8383/0.1617 and 0.7875/0.2125, respectively in three different cattle. χ2 test indicated that the polymorphic sites of intron 3 were in accordance with the Hardy-Weinberg equilibrium (P 0.05) in the three different cattle. The polymorphic sites of exon 3 were in accordance with Hardy-Weinberg equilibrium (P 0.05) in Luxi Yellow cattle and Bohai Black cattle. But it was not in accordance with Hardy-Weinberg equilibrium (P 0.05) in Chinese Holstein cattle.
【Fund】: 国家高科技研究与发展计划(863)项目(No.2006AA10Z1D9);; 国家公益性行业科研专项(No.nyhyzx07-036-09);; 山东省良种工程项目(No.2007LZ10-05)资助
【CateGory Index】: S823
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