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《Journal of Agricultural Biotechnology》 2012-02
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Gene Expression Analysis of Key Enzymes and MYB Transcription Factors in Flavonoid Biosynthesis Pathway during Germination of Fagopyrum tataricum

ZHAO Hai-Xia WU Xiao-Feng BAI Yue-Chen LI Cheng-Lei CHEN Hui SHAO Ji-Rong WU Qi* Department of Life Sciences,Sichuan Agricultural University,Ya'an 625014,China  
As a medicinal and edible plant,tartary buckwheat(Fagopyrum tataricum) is rich in flavonoids especially rutin.During germination,it has a higher concentration of ruin,but the molecular mechanism is still unclear.In this study,the total flavonoids in cotyledon and hypocotyl of F.tataricum during 6~10 days after germination were extracted by the methanol extraction method,and were determined by aluminum chloride spectrophotometry.Semi-quantitative RT-PCR was used to determine the expression level of the phenylalnine ammonialyase gene(Pal),chalcone isomeerase gene(Chi),flavonol synthase gene(Fls) and MYB transcription factor genes(FtMyb1,FtMyb2 and FtMyb3) in the cotyledon and hypocotyl during the seed germination,the relation among them analysed by statistics method.The result showed that:in cotyledons,the total flavonoids content was significantly and positively associated with FtMyb3(0.9625),and negatively with FtMyb2(-0.8572);Chi was significantly and positively associated with FtMyb2(0.8468),and negatively with FtMyb3(-0.8010);the three key enzyme genes were significantly and positively correlated to each other with 0.9119,0.8920 and 0.7584,respectively.In hypocotyls,the total flavonoids content was significantly and negatively associated with Chi(-0.8989),while Fls was significantly negative associated with Chi(-0.7498).Visiblely,the regulation of flavonoid biosynthesis is rather complex during germination of F.tataricum,but some gene's expression is still significantly associated with each other.
【Fund】: 四川省科技厅科技攻关项目(No.2006Z08-012)
【CateGory Index】: S517
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