Control of Tomato Ethylene Biosynthesis by the use of Antisense ACCase Gene
Luo Yunbo; Sheng Jiping(Beijing AgricuItural University Technology Extension,Beijing 100094)Shen Lin(Centre of Beijing Agricultural Technology Extension,Beijing 100101)
Antisense ACCase gene with the 3 5S promoter of CaMV was transfered into leaf discs oftomato plant Lycopersicon esculentum cv. Li Chun)with the help of Ti plasmid of Agrobacterium tumefa-ciens. These leaf discs were placed on solid MS salt medium containing kanamycin and carbenicillin. Sever-al kinds of phytohormones were selected in the medium,and it was found that zeatin and IAA had great ef-fects on the formation of callus and shooting and rooting of transgenic discs. Leaves of these transgenic tomate plants were used for assaying the gene expressinon. Results of5outhern Blot shOwed that antisense ACCase gene was stably integrated into the genome of transgenicplant.The results of RNA hybridization also showed that the target gene had correct expression. Theethylene content of transgenic plant leavf discs was much lower than normal The ripening of transgenictomato was found to be slowed down significantly.The mechanism of regulation of ethylene biosynthesisby the use of ripening-related antisense gene was discussed.
【CateGory Index】： S641.2