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《Agrochemicals》 2013-03
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Simultaneous Determination of 6-Benzylaminopurine and Kinetin Based on D-Tyr Fluorescence Quenching

ZHAO Xiao-hui,ZHU Qian-hua,HE Wei,ZHOU Shang,YANG Qiong,LI Xiao-yan,YANG Ji-dong(College of Chemistry and Chemical Engineering,Yangtze Normal University,Fuling 408000,Chongqing,China)  
The method of simultaneous determination of 6-benzylaminourine and kinetin was established by D-Tyr fluorescence quenching.[Methods] The two natural exogenous cytokinins 6-benzylaminourine and kinetin were analyzed qualitatively by the degree of fluorescence polarization(p) and fluorescence anisotropy(r) based on the measurements of the polarized fluorescence signals,and quantified simultaneously based on the fluorescence quenching spectra signals and the double standard curve method.[Results] The degree of fluorescence polarization and fluorescence anisotropy of the D-Tyr-6-BA system were larger than that of the D-Tyr-KT system,and the two natural cytokinins mixed systems could be studied and characterized via calculating the degree of fluorescence polarization and fluorescence anisotropy.[Conclusions] The method was applied to simultaneous determination of 6-benzylaminourine and kinetin in plum skin and balsam pear skin with satisfactory results.
【Fund】: 国家自然科学基金项目(21175015);; 重庆市教委科技项目(KJ121306)
【CateGory Index】: TQ450.7;O657.3
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