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《PEANUT SCIENCE AND TECHNOLOGY》 2000-01
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Study on RAPD Reaction Conditions for Peanut (Arachis hypogaea L.)

YE Bing-ying 1,CHEN You-qiang 1,ZHU Jin-mao 1,ZHUANG Wei-jian 2 (1. Bio-engineering College,Fujian Teachers' University,Fuzhou 350007,China; 2. Crop College, Fujian Agriculture University)  
The results of RAPD may be influenced by many factors. This paper presents the research results about RAPD reaction conditions for peanut (Arachis hypogaea L). The results suggested that to get good amplified results, the reaction should be performed in a reaction system containing 50mmol/L KCl,10mmol/LTris-HCl (pH9.0),0.1%Triton-100,primer 20～40ng,dNTPs 0.2 nmol/μl,BSA 1～2μg/μl,MgCl 2 15nmol/μl,Taq polymerase 0.5～1 U (Sangon Ltd. products),genomic DNA 30～60ng.
【Key Words】： Arachis hypogaea L. RAPD reaction condition
【Fund】： 福建省教委科学研究基金!(KC1 0 2 1 ) (JB990 0 7)
【CateGory Index】： S565.201
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