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《Journal of Peanut Science》 2002-04
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An Improved Method for RNA Isolation from Peanut Seeds

WANG Fang1,2, ZHANG Bin2, SHAN Lei2, BI Yu-ping2, XU Ze-yong3(1. School of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Plant Biology Technology Research Center, SAAS, Jinan 250100,China; 3. Oil Crops Research Institute ,CAAS,Wuhan 430062,China)  
Peanut seeds are rich in lipid, protein, polysaccharide and phenolic compounds, which increases the difficulties in isolating RNA from them. We compared several methods through experiments and adopted a slightly motified guanidine thiocyanate method. The yield and quality of RNA extracted using this method were high and fit for the standard of many common molecular biology experiments.
【Fund】: 中国农科院油料作物研究所重点开放实验室课题部分实验
【CateGory Index】: S565.2
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【References】
Chinese Journal Full-text Database 10 Hits
1 XIONG Fa-qian;LIU Jun-xian;WANG Cong-cong;HE Liang-qiong;HAN Zhu-qiang;JIANG Jing;ZHONG Rui-chun;LI Zhong;TANG Xiu-mei;TANG Rong-hua;Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences;Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Sciences;Guangxi Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences;Zhongkai University of Agriculture and Engineering;;mCTAB-dLiCl method for efficiently extracting RNA from various tissues of cultivated peanuts and its vertification[J];南方农业学报;2013-11
2 WU Peng;XIU Le-shan;XING Zhao-bin;TIAN Xi-feng;;Comparison of different total RNA extraction methods for Eleutherococcus senticosus[J];中国园艺文摘;2013-11
3 SUN Hai-bo1,2,WANG Zhi-hui1,2,LIU Xue-qun1,HUANG Jun-yan2,DONG Cai-hua2*,LIU Sheng-yi2 (1.College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China; 2.Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China);Modified RNA extraction method for seeds of rapeseed,soybean,peanut and sesame[J];中国油料作物学报;2012-04
4 GAO Xiang-feng WANG Qing-sen (Tea Research Institute of Fujian Academy of Agricultural Sciences,Fu'an Fujian 355015);A Suitable Method of Total RNA Extraction from Tea Leaves for cDNA-AFLP Analysis[J];现代农业科技;2012-06
5 Xie Chuansheng1,2,Song Guoqi2,Wang Xingjun1,2,Xia Han2,Zhao Chuanzhi2,Bi Yuping2(1Science College,Shandong Normal Unveristy,Jinan 250014;2High-Tech Research Center,Shandong Academy of Agricultural Science,Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100);RNA Extraction from Arabidopsis thaliana and Nicotiana tobaccum Immature Seeds using Different Methods[J];中国农学通报;2009-23
6 REN Zeng-kai1,2,YU Shan-lin2,YANG Qing-li2,PAN Li-juan2,CHEN Ming-na2,ZHENG Qiang3(1.College of Plant Technique,Qingdao Agri.University,Qingdao 266109,China;2.Shandong Peanut Research Institute,Qingdao 266100,China;3.Rushan Agricultural Technique Station,Rushan 264500,China);An Efficient Method of Total RNA Extraction from Peanut Seeds[J];花生学报;2008-03
7 HUANG Yue-qin1,2,XU You-ming2,XUE Jian-ping1,3(1.Department of Biology,Huaibei Coal Industry Teachers' College,Huaibei 235000,China;2.College of Horticulture & Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;3.Anhui Key Laboratory of Plant Resources and Biology,Huaibei 235000,China);Efficient method for extraction of high quality RNA from microtubers of Pinellia ternata in vitro[J];中国中药杂志;2008-15
8 Li Cong Zeng Huiming Han Liebao (Institute of Turfgrass Science of BFU,Beijing 100083);Comparative Analyzing of Every Total RNA extract method in Turfgrass[J];生物技术通报;2008-S1
9 Yao Yingqiu1 Chen Xing2 Wang Pu1 Liang Yan2 Yan Mei2 Xiao Bai2(1College of Life Science,Capital Normal University,Beijing 100037;2Beijing Chaoyang Hospital Affiliate of Capital of Medical Sciences,Beijing 100020);Improved Methods for Isolation of High Quality Total RNA from Glycine max Leaves[J];生物技术通报;2008-01
10 Liu Ying,Wang Li,Jiang Yang,Li Sibin,Qi Changqing,Zu Yuangang(Key Laboratory of Forest Plant Ecology of Ministry of Education,Northeast Forestry University,Harbin 150040,P.R.China);A Modification Method of Extracting Total RNA in Compositae Different Tissues[J];东北林业大学学报;2008-01
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Hong 1,2 Wang Xinli 1 1 Laboratory of Plant Biotechnology, Institute of Micobiology, Chinese Academy of Sciences, Bejing, 100080; 2 Department of Light Industry, Hebei Sciences and Technology University, Shijiazhuang, 050018;The Difficulties in the Isolation of RNA from Plant Tissues and their Resolving Strategies[J];生物技术通报;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Xiaoyong;XU Xiaoling;ZHANG Yihui;LIU Yuling;CHEN Xiang;Coll of Hort and Plant Prot,Yangzhou Univ;;Comparison of different methods of total RNA extraction for protoplasts of Citrus[J];扬州大学学报(农业与生命科学版);2016-02
2 YU Keda;YE Meijuan;CHEN Wenrong;ZHU Kaili;ZHANG Changjing;GUO Weidong;College of Chemistry and Life Sciences,Zhejiang Normal University;;Methods for RNA isolation from blueberry tissues[J];浙江师范大学学报(自然科学版);2016-01
3 Kang Yaxuan;Chen Wu;Pang Xiaoming;Li Yingyue;National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plant, Ministry of Education, Beijing Forestry University;;The Comparison and Improvement of RNA Extraction Methods in Different Tissues in Chinese Jujube(Ziziphus jujuba Mill.)[J];分子植物育种;2015-12
4 Chen Xiaofeng;Du Qiuli;Zhao Chunmei;Lv Zhaoyong;Xue Rengao;Qingdao Betterpet Foodstuff Co., Ltd.;Quancheng College, University of Jinan;College of Life Sciences,Qingdao Agricultural University;;Cloning and Expression Analysis of GmCYP78A5 Promoter[J];分子植物育种;2015-12
5 XUAN Jiping;JIA Zhanhui;QIAN Meihua;ZHANG Jiyu;WANG Gang;GUO Zhongren;Zhongshan Botanical Garden,Institute of Botany,Chinese Academy of Sciences;;Comparative of Methods for RNA Extraction From Plum(Prunus salicina Lindl.)Fruit Flesh[J];北方园艺;2015-22
6 ZHANG Yan;HOU Kunlan;Life Science and Technology College,Yangtze Normal University;;Extracting RNA from Leaves of Mustard Brassica Juncea and Measurement and Analysis of Myrosinase Gene[J];长江蔬菜;2015-20
7 WU Ya-yun;ZHANG Shao-peng;ZHAO Xiao-long;SONG Jia;CHEN Ping;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;Lab of Molecular Pharmacology of Traditional Chinese Medicine in Hubei;;Analysis and optimization of high-quality RNA extraction in rhizomes of Panax japonicus C. A. Mey[J];武汉轻工大学学报;2015-03
8 ZHOU Yao-yao;CHEN Chun-yan;CHEN Peng;DIAO Yong;ZHOU Rui-yang;Provincial Key Laboratory of Plant Genetics and Breeding, Guangxi University;;A Method of Extracting High Quality Total RNA from the Anthers and Petals of Kenaf[J];天津农业科学;2015-09
9 LI An-yu;SHE Wen-qin;College of Horticulture,Fujian Agriculture and Forestry University;;Comparison of total RNA extraction methods of olive fruits[J];亚热带农业研究;2015-03
10 ZHANG Yuan-yuan;ZHAO Ji-qiang;JIANG Li-jun;SHI Yan-hui;CHEN Lei;GUO Shan-li;SONG Jian-cheng;Paula E Jameson;School of Life Sciences, Yantai University;School of Biological Sciences, University of Canterbury;;High Quality RNA Isolation from Mature Seeds of Maize Based on Modified and Optimized Protocols[J];玉米科学;2015-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 SUN Hai-bo1,2,WANG Zhi-hui1,2,LIU Xue-qun1,HUANG Jun-yan2,DONG Cai-hua2*,LIU Sheng-yi2 (1.College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China; 2.Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China);Modified RNA extraction method for seeds of rapeseed,soybean,peanut and sesame[J];中国油料作物学报;2012-04
2 XING Zhao-bin,WU Peng,CHEN Long,LIANG Neng-song,HE Shan College of Life Science,Hebei United University,Tangshan 063000,China;Cloning and sequence analyses of Eleutherococcus senticosus GAPDH[J];中草药;2012-01
3 LIU Li-Ming1,2,3, LIU Hong-Yan1,2,3, and TIAN Bao-Ming1 1 Bioengineering Department, Zhengzhou University, Zhengzhou 450001, China; 2 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Henan Key Laboratory for Control of Crop Diseases and Insect Pests, Zhengzhou 450002, China;Selection of Reference Genes from Sesame Infected by Macrophomina phaseo-lina[J];作物学报;2012-03
4 LI Zhi-Feng1,2,3,YANG Jin-huo2,ZHANG Wu-gang1,2,FENG Yu-lin1,2,JIAN Hui1,2,LUO Xiao-Jian1,2,YANG Shi-lin1,2,PEI Yue-hu3 1.National Pharmaceutical Eengineering Center for Solid Preparation in Chinese Herbal Medicine,Nanchang 330006,China 2.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China 3.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;Chemical constituents from Acanthopanax senticosus[J];中草药;2011-05
5 Zong Cheng-Zhi et al(College of Life Sciences,South-central University for Nationalities,Wuhan City,Hubei 430073,China);Analysis of factors affecting RNA isolation qualities[J];广西农学报;2011-01
6 YANG Chen1,2,XU Feng-hua1*,SHAN Shi-hua2*,LIU Yu2,3,LI Chun-juan2, ZHANG Ting-ting2,YAN Cai-xia2(1.Northeast Agricultural University,Harbin 150030,China;2.Shandong Peanut Institute,Qingdao 266100,China;3.Ocean University of China,Qingdao 266100,China);Comparison and Analysis on Extraction Method for Total RNA from Peanut Cotyledon[J];山东农业科学;2011-01
7 Zuo xin-xin,CHEN Ze-yu,MA Teng,YAN Hai-yan,TAN Yan-ping(College of Life Sciences,South-central University for Nationalities,Wuhan,Hubei 430073);A Modified CTAB Method to Extract Total RNA from Animal Tissues[J];中国西部科技;2011-02
8 Chen Gao 1,2 , Shan Lei 2 , Zhou Lixia 1,2 , Tang Guiying 2 , Bi Yuping 1,2 ( 1 Science College, Shandong Normal Unveristy, Jinan 250014; 2 High-Tech Research Center, Shandong Academy of Agricultural Science, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100);The Comparison of Different Methods for Isolating Total RNA from Peanuts[J];中国农学通报;2011-01
9 WANG Ling-ping1,ZHOU Sheng-mao2,,DAI Dan-li1,CAO Jia-shu3(1Institute of Vegetable,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;2 Institute of Vegetable,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;3 Institute of Vegetable,Zhejiang University,Hangzhou 310029,China);Progress in plant phenolic compounds[J];浙江农业学报;2010-05
10 XING Zhao-bin1,LAO Feng-yun2,TIAN Chun-ying1,WANG Jian-shi1(1.Department of Biology,North China Coal Medical College,Tangshan 063000,China;2.Department of Pharmacy,North China Coal Medical College,Tangshan 063000,China);Somatic embryogenesis from petiole in Eleutherococcus senticosus[J];中草药;2009-08
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Wei Shugen;Ma Xiaojun;Fu Jin'e;Zhou Min;Pan Limei;Institute of Medicinal Plant Development, China Academy of Chinese Medical Sciences &Peking Union Medical College;Guangxi Branch of Institute of Medical Plant Development, China Academy of Chinese Medical Sciences;College of Pharmacy, Guangxi University of Traditional Chinese Medicine;;Comparison of Four Methods for Total RNA Extraction from Different Sections of Taxillus chinensis(DC.) Danser[J];世界科学技术-中医药现代化;2016-03
2 Yan Liang;Hai-long Shen;Chun-ping Liu;Ling Yang;Peng Zhang;State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University;School of Life Sciences and A & F, Qiqihar University;;Comparison of methods for extracting high-throughput sequencing RNA from Korean pine seeds[J];Journal of Forestry Research;2016-01
3 LIU Xiangdan;XU Yuqin;WANG Shan;TONG Qiaozhen;ZHOU Ribao;School of Pharmacy, Hunan University of Chinese Medicine;;Screening of Total RNA Extraction Methods For Full-length Gene Cloning from Different Organs in Lonicera macranthoides Hand.-Mazz.[J];湖南中医药大学学报;2015-12
4 XU Yu-qin;WANG Shan;LIU Xiang-dan;TONG Qiao-zhen;ZHOU Ri-bao;SUN Meng-shan;Hu'nan University of Chinese Medicine;;Study on Methods for Extraction of Total RNA from Flower Bud of Lonicera Macranthoides[J];中医药导报;2015-24
5 PENG Bin;WANG Ligang;GU Qinsheng;Henan Key Laboratory of Fruit and Cucurbits Biology;Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences;;Detection of seed- transmitted viruses from dry melon seeds using RT-PCR[J];中国瓜菜;2015-06
6 QIAO Pu;SONG Yu-zhu;ZHANG Jin-yang;CHEN Qiang;HAN Qin-qin;Faculty of Life Science and Technology,Kunming University of Science and Technology;;Efficient Isolation of RNA from Seeds of Tomato and Chili Pepper[J];昆明理工大学学报(自然科学版);2015-05
7 ZHANG Yuan-yuan;ZHAO Ji-qiang;JIANG Li-jun;SHI Yan-hui;CHEN Lei;GUO Shan-li;SONG Jian-cheng;Paula E Jameson;School of Life Sciences, Yantai University;School of Biological Sciences, University of Canterbury;;High Quality RNA Isolation from Mature Seeds of Maize Based on Modified and Optimized Protocols[J];玉米科学;2015-04
8 GENG Xue-jun;FENG Zi-zhou;LI Xiao-jiang;WANG Hai-xia;FENG Lei;KANG Yu;WANG Ya-nan;ZHANG Mei-lin;WU Rui-fang;ZHANG Ji-xing;College of Life Science ,Inner Mongolia University for Nationalities;College of Agriculture,Inner Mongolia University for Nationalities;;Extraction and Quality Analysis of Total RNA from Different Tissues of Alliun Mongulicum Regel[J];内蒙古民族大学学报(自然科学版);2014-06
9 HE Liang-Qiong;XIONG Fa-Qian;TANG Xiu-Mei;JIANG Jing;HAN Zhu-Qiang;ZHONG Rui-Chun;GAO Zhong-Kui;Li Zhong;HE Xin-Hua;TANG Rong-Hua;Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences;Agricultural College of Guangxi University;Guangxi Crop Genetic Improvement and Biotechnology Laboratory;Guangxi Academy of Agricultural Sciences;;Expression Variation of Genes in Early Period of Arachis Artificial Allopolypoidy Evolution Using cDNA-SCoT Technique[J];作物学报;2014-10
10 HOU Jia;SUN Feng-Shuo;WU Qiu-Ming;YANG Yu-Qiao;HE Wei;WANG Yan-Wei;Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University;National Engineering Laboratory for Tree Breeding,Beijing Forestry University;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University;Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University;Forestry Research Institute of Puyang, Puyang;;An Effi cient Method for Total RNA Extraction of Poplar Bark Infected with Pathogen and the Application[J];植物生理学报;2014-02
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