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《Journal of Peanut Science》 2004-04
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Research of Influence Factors on Peanut Transformation Mediated by A. tumefaciens

LI Chun-juan~1, WAN Shu-bo~1, XU Ting-ting~1, ZHUANG Wei-jian~2 , SHAN Shi-hua~1(1.Shandong Peanut Research Institute, Qingdao 266100, China; 2.Crop Scientific College of Fujian A&F University, Fuzhou 350002, China)  
The study has previously transformed the binary antifungle genes, Chitinase and β-1,3-Glucnase gene, mediated with A. tumefaciens into peanut cultivars, Quanhua 10 and Jinhua 1012( Arachis hypogaea L.). The results showed that the regeneration system of three kinds of peanut explants were partially modified and complemented in this research, including advantitious shoots( As), somatic embryogenesis( Se), embryo leaflet( El). Transformation efficiency could be affected by the pre-cultivation time. Transformants have not yet been got through Se and El transformation ways but several scores of Km-resistance buds have been obtained and needed following induction and assaying, and the better transformation efficiency was seen with 10~20 min invasion in A. tumefaciens liquid and 72hr co-cultivation after it. Meanwhile, A. tumefaciens pollution could not be controlled easily when invasion and co-culture time was prolonged. So, how to get higher transformation efficiency but lower pollution ratio should be discussed in the future. In addition, there were some differences between the two used peanut cultivars during transformation.
【Fund】: 农业部"948"(2003 T12)项目;; 山东省农科院博士后基金项目
【CateGory Index】: S565.203
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