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《Chinese Journal of Clinical Rational Drug Use》 2011-17
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Determination of kaempferol in dayinxiang weight reducing tea by HPLC

LIANG Yu-hua,HE Zheng,LIANG Li-chang,et al.The Panyu Central Hospital of Guangzhou City,Guangdong,Guangzhou 511400,China  
Objective To establish a method to determine the content of kaempferol in dayinxiang weight reducing tea so as to control the quality.Methods The HPLC was applied with the condition as follows:chromatographic column:Agilent Hypersil ODS C18(4.6 mm×150 mm,5μm);mobile phase:methanol-0.4%H3PO4(54:46);detection wavelength:367nm;flow speed:1.0 ml/min.Results The HPLC analysis result showed that kaempferol had a good peak profile and separating degree without impurities.The calibration curve for kaempferol was good linear relationship within the range of 0.014~0.280μg with its average sample adding rate of recovery 98.21%(RSD=1.2%).The regression equation was Y=3887.4X+0.6384,r=0.9999.Conclusion The method has a good resolution,strong specificity and good reproducibility,and can be used to determine the content of kaempferol in dayinxiang weight reducing tea.
【CateGory Index】: R286
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