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《Chinese Journal of Pharmaceutics(Online Edition)》 2015-02
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Determination of succinobucol content in nano micelles

BAO Xiao-yue;ZHANG Zhi-wen;QIAO Ming-xi;CHEN Da-wei;School of Pharmacy, Shenyang Pharmaceutical University;Shanghai Institute of Materia Medica, Chinese Academy of Sciences;  
Objectives To establish a method for the determination of the content and entrapment efficiency of succinobucol in nanomicelles. Methods HPLC method was applied using. Discovery-C18 HS F5-5(250 mm×4.6 mm, 5 μm) column. The mobile phase consisted of acetonitrile-water with 0.1% TFA(V:V=80:20). The flow rate was 1.0 m L·min-1 and the detection wavelength was set at 242 nm. The sample volume was 20 μL. Nano micelles and free drug were separated by high speed centrifugation and the entrapment efficiency and drug loading were determined. Results Good linear relationship(r=1.000 0) between peak areas(A) and concentration(ρ) of succinobucol ranging from 1.0-500.0 mg·L-1 was obtained, and the calibration curve was A =1.920 3×104ρ+2.424 6×104. The recoveries were in the range of 98%-102%(n=3) and RSDs were less than 2.0%. The content of succinobucol in nanomicelles was(3.88±0.06) g·L-1. The entrapment efficiency was(96.95±0.02)%. The drug loading was(9.65 ± 0.01)%. Conclusions The method is accurate, simple and rapid, reproducible, and can be used for determination of entrapment efficiency and drug loading of succinobucol in nanomicelles.
【CateGory Index】: R927.2
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Ting-ting,GUO Yuan(Department of Cardiology,The Qilu Hospital of Shandong University,Jinan 250012,China);Advances and Prospects of Probucol[J];Advances in Cardiovascular Diseases;2009-04
2 LIU Li-ping JIN Yang,LI Yi (Department of Pharmacy,College of Chemistry and Chemical Engineering,Shaoxing University,Shaoxing 312000,China);Determination of entrapment efficiency of clotrimazole liposome with Sephadex G-50 gel minicolumn centrifugation-HPLC[J];Chinese Journal of Pharmaceutical Analysis;2007-11
3 FENG Xin1,LI Yao2,WANG Xueying3(1.Deaprtment of Urinary,the First Affiliated Hospital of Liaoning Medical University,Jinzhou 121000,China;2.Department of Physiology,Liaoning Medical University,Jinzhou 121000,China;3.The Jinzhou Central Hospital,Jinzhou 121000,China);Effects of Probucol on Mesangial Cell Proliferation and Extracellular Matrix Production in Rat Induced by Lipopolysaccharides[J];Chinese Journal of Modern Applied Pharmacy;2011-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HU Bo,ZHOU Yu-bin,YANG Yan,YE Xiao-ling,YUAN Zhong-wen,LI Hai-gang,GUAN Shi-xia(School of Chinese Herbal Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China);Determination and entrapment efficiency of docetaxel liposomes for injection by ultrafiltration-HPLC[J];Journal of Guangdong Pharmaceutical University;2012-02
2 LI Ying,ZHU Yu-hong(Department of Neurology,the Second Affiliated Hospital of Kunming Medical College,Kunming 650000,China);Efficacy Observatiom on PAS Therapy for Carotid Atherosclerosis[J];Modern Diagnosis & Treatment;2010-03
3 REN Ning,TANG Hui-fang The First Affiliated Hospital of Nanhua University,Hengyang 421001,Hunan,China;Effects and mechanism of probucol on cardic function in patients with ischemic cardiomyopathy heart failure[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2011-11
4 YUAN Song1,SUN Hui-min1,TU Jia-sheng2,DING Li-xia1(1.National Institute of Food and Drug Control,Beijing 100050,China;2.China Pharmaceutical University,Nanjing 210009,China);RP-HPLC determination of content and entrapment efficiency of indoquinoline tartrate liposomes[J];Chinese Journal of Pharmaceutical Analysis;2011-07
5 ZHANG Quan-lin*,GAO Ling,YAO Hai(Dept.of Pharmacy,Shihezhi People's Hospital,Xinjiang Shihezhi 832000,China);Utilization of Lipid-regulating Agents in Shihezhi People's Hospital During 2008-2010[J];Evaluation and Analysis of Drug-Use in Hospitals of China;2012-01
6 HUANG Jian~(1,2),GU Wangwen~(2*),JIANG Shijun~2,ZHANG Zhiwen~2,CHEN Lingli~2 (1.School of Pharmacy,Jiangsu University,Zhenjiang 212013;2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203);Preparation and Pharmacokinetics in Rats of Probucol Solid Dispersions by Hot-melt Extrusion Process[J];Chinese Journal of Pharmaceuticals;2011-12
7 ZHANG Yang,ZOU Hao,CHEN Jian-ming(College of Pharmacy,Second Military Medical University,Shanghai 200433,China);Determination of entrapment efficiency of teniposide liposomes by Sephadex G-50 gel minicolumn centrifugation-HPLC[J];Chinese Journal of New Drugs;2009-16
8 CUI Teng,GUO Weiying(College of Pharmacy,Liaoning Medical University,Jinzhou 121001);Determination of Entrapment Efficiency of Capecitabine Liposomes by Microcolumn Centrifugation-HPLC[J];Chinese Journal of Pharmaceuticals;2012-08
9 YANG Bo1,DENG Wei1,YAN Tian-ming1,XIE Hong-ping1, JIANG Yun-sheng2,DUAN Shao-bin2(1.Department of Nephrology,the First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,P.R.China;2.Department of Nephrology,the Second Xiangya Hospital,Central South University,Changsha,Hunan 410011,P.R.China);Study of the inhibition effect of Probucol on increased renal impairment to residual renal function by methylguanidine[J];China Journal of Modern Medicine;2013-17
10 WANG Yixin1,JIANG Shijun2,HUANG Jian2,WANG Lingjiao3,SUN Guoxiang1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016;2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203;3.The First Hospital of Hebei Medical University,Shijiazhuang 050000);Effect of Self-assembled Tween 20-SDS Nanometric Mixed Micelles Loaded with Probucol on Improvement of Oral Absorption[J];Chinese Journal of Pharmaceuticals;2013-06
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHAO Shui-Ping,WANG Xing,HONG Shao-Cai,and WU Zhi-Hong(Department of Cardiology,the Second Xiangya Hospital of Central South University,Changsha 410011,China);Effect of Probucol on Heme Oxygenase-1 in Rabbits with Atherosclerosis[J];Chinese Journal of Arteriosclerosis;2008-06
2 YANG Yun Bo, LIAO Duan Fang 1, XIE Zhi Zhong 1, HUANG Hong Lin 1 (Dept of Pharmacology, Institute of Pharmacy,Traditional Chinese Medical College,Changsha 410007);Probucol prevention against restenosis by regulating functional vascular remodeling after percutaneous transluminal angioplasty in rabbits[J];Chinese Pharmacological Bulletin;2003-04
3 Liu debao,Bu haifu,Gui binjie (Dept of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230022);Experimental study on prevention of hormonally ischemic necrosis of femoral head in rabbits with combined use of Probucol and Aspirin[J];Acta Universitatis Medicinalis Anhui;2006-01
4 JIA Wei-hua,ZHOU Li-chun,TIAN Gui-ling(1 Department of Neurology,Beijing Chaoyang Hospital(West Campus),Capital University of Medical Sciences,Beijing 100043,China;2 Department of Neurology,General Hospital of Tianjin Medical University,Tianjin 300070,China);Clinical evaluation of probucol for the treatment of plaque in carotid artery[J];Chinese Journal of New Drugs;2007-03
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