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《Journal of Ocean University of Qingdao》 2005-06
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Nucleotide Analyzing Techniques Applied in Molecular Systematics of Red Algae

ZENG Xiao-Qi~1,HU Zi-Min~1,DUAN De-Lin~2(1.Ocean University of China,Qingdao 266003,China;2.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)  
The acquisition and analysis of nucleotide sequence data is crucial to molecular systematics study of red algae,because it is an important evolutionary unit with site mutation,which represents the difference of interspecies or introspecies and reflects the process and frequency of evolution.Presently,the nucleotide markers used for systematic and evolutionary studies mainly include restriction fragment length polymorphism(RFLP),random amplified polymorphism DNA(RAPD),amplified fragment length polymorphism(AFLP) and intersimple sequences repeats(ISSR).The sequences for nucleotide analyzing include internal transcribed spacer(ITS),18S and 28S rRNA,ribosomal large and small subunit(LSU and SSU),rubisco gene and flanked large and small subunit in chloroplast and mitochondria.So far,these techniques have been widely utilized in the study of species identification,populational genetic and differentiation,taxonomy,systematics and evolution of red algae.
【Fund】: 国家高技术研究发展计划(2001AA621090);; 中科院海洋所实验海洋生物学重点实验室开放课题资助
【CateGory Index】: Q949.29
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