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《Periodical of Ocean University of China》 2007-S2
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Optimization of Culture Conditions for Transgenic Synechocystis sp.PCC6803

ZHANG Xiao-Qing,ZHANG Xue-Cheng,ZANG Xiao-Nan(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China)  
By examining the effects of nitrate,phosphate and carbonate contents in the medium on the growth of transgenic Synechocystis sp.PCC6803 with paralichthys olivaceus growth hormone gene,we conducted experiments through orthogonal tests based on the results of single factor experiments in order to obtain the optimal medium conditions.We used the same method of orthogonal test based on the optimal medium conditions to achieve the best environment factors.The results of our experiment demonstrated that the optimal medium conditions for transgenic strain based on BG-11 medium were determined as follows: The NaNO3,K2HPO4 and Na2CO3 concentrations were 1.25 g/L,0.04 g/L and 0.03 g/L(The rest of the components were the same as BG-11 medium).The optimal culture conditions were temperature 30 ℃,light intensity 3 000 lx and pH 8.0.Under these optimal culture conditions,the biomass of the transgenic alga increases quickly and the specific growth rate of the transgenic Synechocystis sp.PCC6803 reached 20.64.This paper lays an academic foundation for achieving high density and amplificatory culture of transgenic Synechocystis sp.PCC6803.
【Fund】: 国家高技术研究发展规划项目(819-04-10);; 农业转基因生物安全管理办公室批准项目(2005-T104)资助
【CateGory Index】: Q943.2
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