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《Periodical of Ocean University of China》 2014-01
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Cloning of Imd Immune Signal Pathway Relating Genes of Fenneropenaeus chinensis and Their Expression Analysis

GE Qian-Qian;LI Jian;LIANG Jun-Ping;LIU Ping;ZHAO Fa-Zhen;PAN Lu-Qing;LIU De-Yue;Ocean University of China;Yellow Sea Fisheries Research Institute,Chinese Acedemy of Fishery Sciences;  
Fenneropenaeus chinensis is an important mariculture species in China.Recently,epidemic prawn disease as the main factor has limited its sustainable and healthy culture.Invertebrates use their innate immunity as the the first barrier against pathoghens.The inducible antimicrobial peptides(AMPs) regulated by Toll and immune deficiency(Imd)pathway are effectors of Drosophilainnate immunity,and Imd pathway responds to the infection of Gram-negative bacteria.In this study,we cloned an Imdgene fromF.chinensis,named FcImd.The full-length cDNA of FcImd was 783bp,consisting of an ORF of 483bp,which encoded putatively aprotein of 160amino acids with a death domain at its C-terminal.Homology analysis revealed that FcImd was highly conservative among crustaceans.The similarity of FcImd with that of Litopenaeus vannamei and Marsupenaeus japonicus were 99%and 88%,respectively.Phylogenetic analysis showed that FcImdclustered with insect IMDs and vertebrates RIP1s.The hemocytes were drawn at 1,3,6,12,24,48,96and 144hafter being challenged with Vibrio anguillarum.The expression level of FcImdin hemocytes was analyzed with quantitative real-time PCR.It was found that the expression of FcImdand Relish was up-regulated to different extents.Our findings implied that Imd immune signal pathway may play an important role in prawn immune response.
【Fund】: 国家虾产业技术体系项目(CARS-47);; 国家高技术研究发展计划项目(2012AA10A409);; 公益性行业(农业)科研专项费用项目(201103034)资助
【CateGory Index】: S917.4
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