Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Periodical of Ocean University of China》 2014-01
Add to Favorite Get Latest Update

Cloning of Imd Immune Signal Pathway Relating Genes of Fenneropenaeus chinensis and Their Expression Analysis

GE Qian-Qian;LI Jian;LIANG Jun-Ping;LIU Ping;ZHAO Fa-Zhen;PAN Lu-Qing;LIU De-Yue;Ocean University of China;Yellow Sea Fisheries Research Institute,Chinese Acedemy of Fishery Sciences;  
Fenneropenaeus chinensis is an important mariculture species in China.Recently,epidemic prawn disease as the main factor has limited its sustainable and healthy culture.Invertebrates use their innate immunity as the the first barrier against pathoghens.The inducible antimicrobial peptides(AMPs) regulated by Toll and immune deficiency(Imd)pathway are effectors of Drosophilainnate immunity,and Imd pathway responds to the infection of Gram-negative bacteria.In this study,we cloned an Imdgene fromF.chinensis,named FcImd.The full-length cDNA of FcImd was 783bp,consisting of an ORF of 483bp,which encoded putatively aprotein of 160amino acids with a death domain at its C-terminal.Homology analysis revealed that FcImd was highly conservative among crustaceans.The similarity of FcImd with that of Litopenaeus vannamei and Marsupenaeus japonicus were 99%and 88%,respectively.Phylogenetic analysis showed that FcImdclustered with insect IMDs and vertebrates RIP1s.The hemocytes were drawn at 1,3,6,12,24,48,96and 144hafter being challenged with Vibrio anguillarum.The expression level of FcImdin hemocytes was analyzed with quantitative real-time PCR.It was found that the expression of FcImdand Relish was up-regulated to different extents.Our findings implied that Imd immune signal pathway may play an important role in prawn immune response.
【Fund】: 国家虾产业技术体系项目(CARS-47);; 国家高技术研究发展计划项目(2012AA10A409);; 公益性行业(农业)科研专项费用项目(201103034)资助
【CateGory Index】: S917.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 4 Hits
1 LI Li,YAN Yan(Dermatology Dept.of Peking Union Medical College Hospital,CAMS & PUMC,Beijing 100730,China);Progress of study on functions of RIP1 in cell signal transduction[J];Basic & Clinical Medicine;2011-10
2 KANG Cui-jie 1,WANG Jin-xing 1,ZHAO Xiao-fan 1& XIANG Jian-hai 2 ( 1)School of Life Sciences,Shandong University,Jinan 250100,China; 2)Institute of Oceanology,The Chinese Academy of Sciences,Qingdao 266071,China);Molecular Cloning of the Cdna Encoding a Mature Antimicrobial Peptide from Shrimp,Penaeus Chinensis[J];Journal of Shandong University(Natural Science);2002-06
3 LI Zhong_hai,DUAN MU De_qiang,WANG Jing_ze (State Key Laboratory of Biomembrane and Membrane Biotechnology,Institute of Zoology, The Chinese Academy of Sciences,Beijing,100080,China);The signalling pathway and physiological functions of activation of NF-κB[J];Joural of Biology;2002-04
4 GUO Zhi-xun,FENG Juan,WANG Jiang-yong(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China);Clearance of Vibrio anguillarum by haemocytes in giant black tiger shrimp Penaeus monodon in vivo[J];Journal of Fishery Sciences of China;2006-01
Chinese Journal Full-text Database 10 Hits
1 LI Guang1,2,FAN Jing-feng1,LIN Feng-ao1,LIANG Yu-bo1(1.National Marine Environmental Monitoring Center,Dalian 116023,China;2.Dalian Fisheries University,Dalian 116023,China);Research Progress on Immune Mechanism and Immunoprophylaxis of Diseases in Shrimp[J];Fisheries Science;2007-01
2 ZHANG Hua-jun1,2,LI Zhuo-jia1,ZHANG Jia-song1,GUO Zhi-xun1,WEN Guo-liang1,CAO Yu-cheng1,WU Yin2 (1.School of Life Science and Technology,Dalian Ocean University,Dalian 116023,China;2.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China);Changes in non-specific immune parameters and water quality in Pacific white leg shrimp Litopenaeus vannamei culture ponds[J];Journal of Dalian Ocean University;2011-04
3 AN Xian-hui 1,2, LV Yi1, LI Wei-guo2, LIANG Jian-guo1, XU Chun-hua1,ZHANG Chong-xing1, DONG Jing1, LAI Ren 1,3,*(1. College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;2. Key of Oceanic Biotechnology Lab. of Jiangsu, Ocean College,Huaihai Institute of Technology, Lianyungang, Jiansu 222005, China;3. Department of Animal Toxicology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China);Antimicrobial Peptides Purified from Penus chinensis[J];Zoological Research;2005-04
4 LIANG Ai-ling1,2,LIU Yong-jun2,HOU Gan1,2,HUANG Di-nan1,2 1.Institute of Laboratory of Medicine,Guangdong Medical College,Dongguan 523808,China 2.Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Dongguan 523808,China;Screening for antimicrobial peptide genes in scallop by improved PCR method of rapid amplification of 3' cDNA ends[J];Drugs & Clinic;2011-05
5 Shen Guiyu;;Reseach advance in the effect of some major pollution factors on the health of prawn and crab[J];Feed Industry;2013-22
6 XUE Jian-Feng, KANG Cui-Jie, WANG Jin-Xing, ZHAO Xiao-Fan, XIANG Jian-Hai (School of Life Sciences, Shandong University, Jinan, 250100) (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071);RECOMBINANT EXPRESSION OF CH-PENAEIDIN FROM CHINESE SHRIMP FENNEROPENAEUS CHINENSIS IN ESCHERICHIA COLI[J];Oceanologia Et Limnologia Sinica;2005-03
7 HUANG Bing-Xin1,2,JIANG Hao1,2,ZHANG Ji-Quan1,XIANG Jian-Hai1(1.Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071;2.Graduate School,Chinese Academy of Sciences,Beijing,100049);PROFILING THE PLASMA PROTEOME OF FENNEROPENAEUS CHINENSIS WITH SDS-PAGE AND LC-MS/MS[J];Oceanologia Et Limnologia Sinica;2009-02
8 GUO Zhen-yu, DONG Bo, JIAO Chuan-zhen, XIANG Jian-hai(Laboratory of Experimental Marine Biology, Institute of Oceanology, the Chinese Academy of Sciences, Qingdao 266071, China);Expression of antimicrobial peptide during the growth period of shrimp Fenneropenaeus chinensis[J];Marine Sciences;2004-01
9 LU Qi-neng,LUO Xiao-ming,JIANG Hui,ZOU Yi-ping(School of Life Sciences,Resources and Environment Sciences,Yichun University,Jiangxi Yichun 336000 PRC);Progress in Investigation of Antimicrobial Peptides[J];Jiangxi Science;2007-04
10 LIANG Ai-ling1,2,LIU Yong-jun2,HOU Gan1,2,HUANG Di-nan1,2(1.Institute of Laboratory of Medicine;2.Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Dongguan,Guangdong 523808,China);Study of screening for defensin genes in scallop by improved rapid amplification of cDNA ends[J];Laboratory Medicine and Clinic;2012-10
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Chunyi Zhang Yunliu Fan (Biotechnology Research Center, CAAS, Beijing 100081);Expression of Anti fungal Tachyplesin Gene in E.coli[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1998-03
2 Xu Bing Ji Weishang Xu Huaishu(Department of Marine Biology,Ocean University of Qingdao)Shi Jie(Institute of Dermatology,Qingdao Medical College);THE IMMUNOLOGIC COMPETENCE OF WHEAT GERM AGGLUTININ IN PENAEUS ORIENTALIS[J];Journal of Ocean University of Qingdao;1992-04
3 Ye Yinling Chen Kuanzhi(Department of Biology);STUDIES ON THE CIRCULATING HEMOCYTES OF PENAEUS CHINENSIS[J];Journal of Ocean University of Qingdao;1993-02
4 LI Jian Min, ZHOU Kai Ya, ZHANG Shuang Quan, DAI Zhu Ying(Department of Biology, Nanjing Normal University, Nanjing 210097, China);ZHU Chang Liang,YE Xin Hai(Department of Biology, Nanjing Medical University, Nanjing 210029, China).;Sequencing Partial Fragment of Cecropin A cDNA in the Silkworm Bombyx mori From China.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1999-04
5 Shen Junqing Qu Xianming 1(Shanghai Research Center of Biotechnology,The Chinese Academy of Science,Shanghai 200233) Tian Yu 2(Department of Molecular Genetics,Shanghai Medical University,Shanghai 200032);Cloning and Expression of Antibaterial Peptide ABP3 Gene in Pichia pastoris Project of Chinese National Programs for High Technolgy Research and Development(No.101 01 02 03).[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1999-04
6 Chen Liuchun Wang Jinxing( Departm entof Biology, Schoolof Life Sciences, Shandong University, Jinan 250100);The Antibacterial Peptites From Insects[J];PROGRESS IN BIOTECHNOLOGY;1999-05
7 Du Aifang Cai Weiming Yu Lian(Zhejiang Agricultural University ,Hangzhou 310029);PHAGOCYTOSIS OF PENAEUS CHINENSISHAEMOCYTES[J];JOURNAL OF FISHERY SCIENCES OF CHINA;1997-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved