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《Practical Journal of Medicine & Pharmacy》 2006-10
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Expression of fungal immunomodulate protein(LZ-8)in E.coli and its antibody preparation in rabbits

BAI Jie-ying~(①②) ZENG Lin LI Yan-fang①Plant Science School of Agriculture Center,Jilin University,Changchun 130062,China  
Objective Through the expression of fungal immunomodulate protein(LZ-8)in E.coli to get satistactory antibody.Method The LZ-8 gene was cloned into the vector pET-30a-lz8 and expressed in E.coli BL21,which was induced by IPTG;the total protein of induced by E.coli was purified using Ni-NTA,then the purified protein was used to make polyclonal antibody by routine method,the antigenieity was detected by ELISA.Results Above-mentioned expressed protein(about 14ku)amount to 34.46mg/L according to Coomassie Brilliant;ELISA detection showed that the protein had a satisfactory antigenicity,which was diluted about 104 fold.Conclusion Fungal immunomodulate protein(LZ-8)can be expressed in E.coli,and the high satisfactory antigenicity polyclonal antibody is obtained from rabbit,thus providing basis of following research on this protein.
【CateGory Index】: R392;Q789
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