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《Organ Transplantation》 2012-05
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Impact of retrograde perfusion in autologous orthotopic liver transplantation on acute lung injury in rats

SU Zhao-jie, JIANG Yi, LV Li-zhi.Clinical Medical School of Fuzhou General Hospital of Nanjing Military Area, Fujian Medical University, Fuzhou 350025, China  
Objective To explore the impact of retrograde perfusion via inferior vena cava and antegrade perfusion via portal vein in autologous orthotopic liver transplantation on acute lung injury in rats. Methods Seventy-five Sprague-Dawley(SD) rats were randomly divided into three groups: retrograde perfusion group, antegrade perfusion group and sham operation group. After the models were established, six rats in each group were randomly executed at 6 h, 12 h and 24 h after operation,respectively. Dry-to-wet weight ratio of lung, the activity of myeloperoxidase(MPO), the content of malondialdehyde(MDA) and tumor necrosis factor(TNF)-α of lung tissue were measured. And the pathological section of lung was observed. Results Compared with sham operation group, dry-to-wet weight ratio of lung was lower in retrograde perfusion group and antegrade perfusion group, while the activity of MPO, the content of MDA and TNF-α were higher in the two groups. They were highest especially at 6 h and 12 h after operation. Compared with antegrade perfusion group, dry-to-wet weight ratio of lung was higher in retrograde perfusion group at 6 h and 12 h after operation, however, the activity of MPO and content of MDA in lung tissue were lower, in which there were significant difference(all in P0.05). There was no significant difference between two groups at 24 h after operation(all in P0.05). The content of TNF-α in retrograde perfusion group was lower at 6, 12 and 24 h after operation, and there was significant difference between two groups (all in P0.05). The result of hematoxylin-eosin(HE) staining of lung issue showed different levels of performances in retrograde perfusion group and antegrade perfusion group, including destruction of alveolar structure, thickening of alveolar interval, congestion of small vein and capillary, and infiltration ofinflammatory cells. The performance at 6 h and 12 h was more severe than that at 24 h after operation. And the pathological damage of lung tissue in retrograde perfusion group was obviously milder than that in antegrade perfusion group. Conclusions Acute lung injury occurs in the early period both retrograde perfusion or antegrade perfusion in autologous orthotopic liver transplantation in rats, and the damage is more serious at 6 h and 12 h and alleviated at 24 h after operation. The level of acute lung injury in retrograde perfusion is lower than that in antegrade perfusion.
【Fund】: 福建省自然科学基金重点项目(2011Y0046)
【CateGory Index】: R657.3
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