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《Chinese General Practice》 2012-18
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Effect of Phillyrin on Gene Expression of Influenza A Virus Nucleoprotein

DUAN Lin-jian,ZHANG Qing,WANG Nong-rong,et al.Medical College of Nanchang University,Nanchang 330006,China  
Objective To investigate the effect of phillyrin on gene expression of influenza A virus nucleoprotein after transfection.Methods Hela cells were transfected with NP gene of influenza A virus,and Influenza A virus colloidal gold method was used to detect the phillyrin supernatant and intracellular nucleoprotein expression,and RT-PCR was used to detect the NP gene copy number.Results NP recombinant plasmid group contained high level influenza A virus nucleoprotein.Empty plasmid group,liposome group and Hela cells group mainly did not contain influenza A virus nucleoprotein.Phillyrin supernatant contained no or little influenza A virus nucleoprotein.Phillyrin contained low level intracellular influenza A virus nucleoprotein.RT-PCR calibration curve showed the correlation was 0.998 and the efficiency was 97.4%.48 h after four times transfection,NP gene expression level was(2.05±0.3)×105 copy number/μl in Phillyrin group,(61.5±15.0)×105 copy number/μl in recombinant plasmid group,significantly higher than Phillyrin group.The difference of NP gene expression level between Phillyrin group and recombinant plasmid group was statistically significant(t=7.672,P0.O5).Conclusion Phillyrin can inhibit the expression of Influenza A virus NP gene after transfection.
【Fund】: 国家自然科学基金项目(30860351);; 江西省卫生厅中医药科研基金(2008ZZ0014)
【CateGory Index】: R285
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