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Correlation between anti-tumor activity of quercetin and the inhibitive effect on Wnt/β-catenin signaling pathway

LI Run-qing1,SHAN Bao-en21.Center of Clinical Laboratory,Affiliated Beijing Youan Hospital of Capital Medical University,Beijing 100069,P.R.China2.Research Center,Affiliated Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,P.R.China  
OBJECTIVE: To investigate the relationship between the anti-tumor activity of quercetin and the inhibition of Wnt/β-catenin signaling pathway.METHODS: SW480 cells were incubated with quercetin at several concentrations and for different time.The MTT colorimetric assay was used to examine the effect of quercetin on the proliferation of SW480 cells.Reporter gene plasmid TOPflash or FOPflash was cotransfected into SW480 cells together with pRL-null plasmid,and the reporter gene assay was performed to detect the changes of the transcriptional activity of β-catenin /TCF in SW480 cells after quercetin was added.RT-PCR and Western blot analysis were performed to examine the transcriptional level and protein expression level of β-catenin target genes,Cyclin D1 and Survivin.RESULTS: The proliferation of SW480 cells was inhibited significantly by quercetin within the concentrations from 40 to 160 μmol/L,P0.01,showing a dose-and time-dependent manner.The TCF-4 reporter gene activity in SW480 cells was inhibited significantly by quercetin within the concentrations from 10 to 160 μmol/L,P0.05,and mRNA and the protein expression of β-catenin/TCF target genes,Cyclin D1 and Survivin in SW480 cells were also down-regulated markedly by quercetin,in a dose-dependent manner.CONCLUSION: Quercetin can inhibit the proliferation of SW480 cells in vitro and the anti-tumor mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.
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