Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer Prevention and Treatment》 2008-21
Add to Favorite Get Latest Update

Correlation between anti-tumor activity of quercetin and the inhibitive effect on Wnt/β-catenin signaling pathway

LI Run-qing1,SHAN Bao-en21.Center of Clinical Laboratory,Affiliated Beijing Youan Hospital of Capital Medical University,Beijing 100069,P.R.China2.Research Center,Affiliated Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,P.R.China  
OBJECTIVE: To investigate the relationship between the anti-tumor activity of quercetin and the inhibition of Wnt/β-catenin signaling pathway.METHODS: SW480 cells were incubated with quercetin at several concentrations and for different time.The MTT colorimetric assay was used to examine the effect of quercetin on the proliferation of SW480 cells.Reporter gene plasmid TOPflash or FOPflash was cotransfected into SW480 cells together with pRL-null plasmid,and the reporter gene assay was performed to detect the changes of the transcriptional activity of β-catenin /TCF in SW480 cells after quercetin was added.RT-PCR and Western blot analysis were performed to examine the transcriptional level and protein expression level of β-catenin target genes,Cyclin D1 and Survivin.RESULTS: The proliferation of SW480 cells was inhibited significantly by quercetin within the concentrations from 40 to 160 μmol/L,P0.01,showing a dose-and time-dependent manner.The TCF-4 reporter gene activity in SW480 cells was inhibited significantly by quercetin within the concentrations from 10 to 160 μmol/L,P0.05,and mRNA and the protein expression of β-catenin/TCF target genes,Cyclin D1 and Survivin in SW480 cells were also down-regulated markedly by quercetin,in a dose-dependent manner.CONCLUSION: Quercetin can inhibit the proliferation of SW480 cells in vitro and the anti-tumor mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.
【CateGory Index】: R730.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 TANG Lei,YANG Yang,YIN Jiang-xia,ZHAO Chang-lin Department of Oncology,Xinhua Hospital Affiliated to Dalian University,Dalian 116021,P.R.China;Correlation between Survivin,β-catenin and recurrence,metastasis of rectal cancer[J];Chinese Journal of Cancer Prevention and Treatment;2010-11
Chinese Journal Full-text Database 2 Hits
1 MA Shuang-wei,WU Kai-nan,ZHONG Xiao-gang,KONG Ling-quan Department of General Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,P.R.China;Study of the inhibitory effect of quercetin on transplantation tumor of breast carcinoma cell line MCF-7 in nude mice[J];Journal of Qilu Oncology;2004-03
2 Zhang Jie Lü Cai Zhang Ping-an et al Department of Urology, People's Hospital of Wuhan University, Wuhan;Empirical Studies on Quercetin for Inhibition of Proliferation ofhuman Uterine Cervix Cancer HeLa cells[J];Chinese Journal of Clinical Oncology;2006-08
Chinese Journal Full-text Database 9 Hits
1 XIE Fu-Hua1,2,WANG Run-Xiu3,LI Li-Ping2,QIN Yan-Mei2,LIANG Nian-Ci2 1Department of Biochemistry and Molecular Biology,3Department of Nephronia,First Affiliated Hospital,Gannan Medical College,Ganzhou 341000,China,2Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang 524023,China;Anti-tumor action of adenovirus mediated Survivin RNAi in human breast cancer-bearing nude mice[J];Journal of the Fourth Military Medical University;2008-19
2 HUANG Jing,ZHANG Wei,LI Fumin,LI DanDept.of Gynecology and Obstetrics,Renmin Hospital of Wuhan University,Wuhan 430060,China;Effect of Quercetin on the Expression of Nm23 Gene in HeLa cells[J];Medical Journal of Wuhan University;2009-05
3 HE Jian-bin, ZHANG Ping, OU Li-wen, WANG Xiao-hua Department of Respiration, First Affiliated Hospital of Nanhua University, Hengyang 421001, P.R.China;Expressions of HIF-1α and P-Selectin induced by Suramin combination with Quercetin in lung adenocarcinoma in mice[J];Chinese Journal of Cancer Prevention and Treatment;2006-11
4 Zhang Yonggang,Zhang Jie,Fan Hong,et al.Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Effect of Quercetin on the expression and growth of Bcl-2 in lung adenocarcinoma cancer cell line A549.[J];Sichuan Medical Journal;2007-10
5 Wang Haiyan Wang Gaofagn Lin Gan Yu HaizhongDepartment of Chemistry and Biologic Science,Xiangfan College(Xiangfan Hubei 441053,China);Effect of Chinese Herbal Medicine on the Cell Cycle of Hela Cells[J];Journal of Sichuan of Traditional Chinese Medicine;2007-08
6 Pharmacological School, Jinan University XU Yan CAI Yu LIANG Shao-ling YANG Yan-xia;Research Advance of Anti-tumor Materia Medica Acting on Cellular Message Transmission Pathway[J];Shanghai Journal of Traditional Chinese Medicine;2005-08
7 WANG Chong1,2,TAN Sai-nan2,3,LU Cai-ling2,YAO Nan1,2,MA Xu2(1Graduate School of Peking Union Medical College,Beijing 100730,China;2Dept of Genetics,National Research Institute for Family Planning;3Family Planning Institute of Hunan Province);The research of quercetin on tumor suppression of conlon cancer[J];Journal of Shanxi Medical University;2009-06
8 BAI Ji-qing,XI Xiao-xian,HE Xin-huai(Shaanxi University of TCM,Xianyang 712046,Shaanxi,China);Researches of Polysacchaides from Spores of Agaricusbaizei Murill Inhibiting Transplanted Tumor in the Nude Rats[J];Chinese Archives of Traditional Chinese Medicine;2007-10
9 Yin LP LI G Fang G;Progress on the Experiment Study of Mechanism of Action of TCM Bioactive Component Induced HeLa Cell Apoptosis[J];Chinese Medicine Modern Distance Education of China;2008-11
Chinese Journal Full-text Database 1 Hits
1 Liu Feng Sun Lingyu Zhao Liang et al Department of Abdominal Surgery,the Tumor Hospital of Harbin Medical University,Harbin;The Expression and Correlation between β-catenin and Survivin in Rectal Carcinoma[J];Chinese Journal of Clinical Oncology;2007-13
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Kong Lingquan Wu Kainan Lin Hui Department of Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing;Study of Inhibitory Effect of Quercetin on Neovascilarization of Experimental Mammary Carcinoma[J];Chinese Journal of Clinical Oncology;2001-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved