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《Chinese Journal of Cancer Prevention and Treatment》 2009-05
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Inhibitory effect of YC-1 on stability and transcriptional activity of HIF-1 in hypoxic human liver cancer cells-HepG-2

DU Jing1,ZHAO Qiu21.Department of Gastroenterology,Zhejiang Provincial People's Hospital,Hangzhou 310014,P.R.China2.Department of Gastroenterology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,P.R.China  
OBJECTIVE:To investigate the effects of YC-1 on stability and transcriptional activity of HIF-1 in human liver cancer cells-HepG-2 incubated in hypoxic conditions,and to discuss probable mechanism which there is.METHODS:Human liver cancer cells-HepG-2 were incubated under hypoxic culture.Semi-quantitative RT-PCR and Western blotting were used to detect the HIF-1α and its target genes' mRNA and protein levels treated with diverse concentrations of YC-1.MTT assay were used to detect proliferation vitality of hypoxic HepG-2 cells.RESULTS:Comparing to the normoxic controls,for HIF-1α and its target genes,the mRNA levels highly increased in hypoxia,P0.05.Under hypoxic conditions,the mRNA synthesis and protein expressions of those targeted genes decreased progressively after dealt with increasing dose of YC-1(P0.05),and YC-1 seemed to have no effect on the HIF-1α mRNA levels,but it developed a dose-dependent reduction on HIF-1α protein expression.YC-1 inhibited the proliferation of hypoxic HepG-2 cells in a dose-dependent manner.CONCLUSIONS:YC-1 inhibits the stability and transcriptional activity of HIF-1 in a dose dependent-manner in human liver cancer cells-HepG-2 which is incubated in hypoxia.YC-1 inhibits the proliferation of hypoxic HepG-2 cells in a dose-dependent manner.Our study suggests that HIF-1 might become a new potential therapeutic target for human liver cancer.
【CateGory Index】: R735.7
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Huan-fen, ZHANG Yong-mei, HE Chun-nian, SONG Guang-yao, ZHAI Jin-ping Hebei General Hospital, Shijiazhuang 050051, P.R.China;Relation between tumor drug resistance and expression of hypoxia induced factor 1α in lung carcinoma[J];Chinese Journal of Cancer Prevention and Treatment;2008-07
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1 WANG Cheng1,LI Bao-zhong2,WAN Jun-ting2,TAN Xiao-gang2,QIU Bin2,XING Xue-zhong2,SHAO Kang2,HE Jie2,ZHANG You-cheng1 1.Department of Thoracic Surgery,Second Hospital of Lanzhou University,Lanzhou 730030,P.R.China 2.Department of Thoracic Surgery,Cancer Hospital (Institute),Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing,100021,P.R.China;Expression and correlation with prognosis of p190-B RhoGAP in patients with lung adenocarcinoma[J];Chinese Journal of Cancer Prevention and Treatment;2009-19
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