Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer Prevention and Treatment》 2009-07
Add to Favorite Get Latest Update

Effect of RNA interference targeting at peroxisome proliferator-activated receptor gamma gene on angiogenesis of hepatocellular carcinoma in nude mice

WU Xu-dong1,CHEN Wei-chang1,ZHU Shu-yang2,YE Jian-xin1 1.Department of Gastroenterology,First affiliated Hospital of Soochow University,Suzhou 215006,P.R.China2.Department of Pediatrics,North Courtyard of First People's Hospital of Chenzhou City,Chenzhou 423000,P.R.China  
OBJECTIVE:To investigate the effect of short hairpin RNA(shRNA)targeting at peroxisome proliferator-activated receptor gamma(PPARγ)gene on angiogenesis of hepatocellular carcinoma and its mechanicm in nude mice.METHODS:shRNA targeting at PPARγ gene was constructed and transfected into HCCLM3 cells(pshPPARγ group),meanwhile,the cells transfected with vector plasmids were as a control group.Positive clones were selected and the transplanted tumor animal models constructed in nude mice,and the growth and volume of tumors were observed.After 8 weeks,PPARγ mRNA and protein in transplanted tumor tissues were detected,and pulmonary metastases were counted and graded.Moreover,Anti-CD34,VEGF and TSP1 were stained by the immuocytochemistry method,and MMP-2 and TIMP2 mRNA were detected by RT-PCR.RESULTS:PPARγ mRNA was down regulated significantly in the pshPPARγ group,and its inhibition rate was 82%.In the pshPPARγ group,the tumor volume was(1.86±0.65)cm3,MVD was 20.84±6.38,and pulmonary metastases were at grade 1-2 and 37.2±0.7 per lung;while in the control group,tumor volume was(4.86±1.15)cm3,MVD was 39.48±9.01,and pulmonary metastases were at grade 3-4 and 107.8±6.1 per lung,In the pshPPARγ group,TSP-1 protein presented positive,and VEGF protein presented weakly positive or negative.The expression of MMP-2 mRNA decreased,TIMP2 mRNA increased in the pshPPARγ group,and there were significant differences compared with the control group,t=11.34 or 8.44,P0.01.CONCLUSION:RNA interference targeting at PPARγ gene can balance TSP1/VEGF,through regulating the expression of MMP-2/TIMP2 to impair angiogenesis of HCC in nude mice.
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Lu Jing1,Wang Bin1,Mi Mantian1,Zhang Qianyong1,Zhu Jundong1,Wang Jian2 (1Department of Nutrition and Food Hygiene,College of Military Preventive Medical,Research Center for Nutrition and Food Safety,Third Military Medical University,Chongqing,400038; 2Department of Nutrition,Xinqiao Hospital,Third Military Medical University,Chongqing,400037,China);Expression and significance of lipid metabolism-related genes in breast invasive ductal carcinoma[J];Acta Academiae Medicinae Militaris Tertiae;2010-12
Chinese Journal Full-text Database 6 Hits
1 LI Gong-jie, YANG Li, SHI Xiao-lin, LI Xiao-bin, ZHEN Xiao-ling, WANG Yue-hua. Radiology Department of 307 Hospital of PLA, Beijing 100039, China;Study of the relationship between morphological characteristics of tumor microvasculature and blood supply type of primary hepatocellular carcinoma observed under DSA[J];Journal of Interventional Radiology;2005-02
2 CAO Ji1,2,YANG Chun1,LI Li-ping1,CHEN Jun1,OU Chao1,DUAN Xiao-xian1,LI Yuan1,SU Jian-jia1,YUE Hui-fen11.Department of Experimental Research,Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,P.R.China2.Medical Scientific Research Center of Guangxi Medical University,Nanning Shuobo Biological Technology CO.,Ltd.Nanning 530021,P.R.China;Expressions and clinical significance of CD146 and MMP-2 in hepatocellular carcinoma[J];Chinese Journal of Cancer Prevention and Treatment;2007-10
3 WU Xudong,CHEN Weichang,ZHANG Suqing.Department of Gastroenterology,Affiliated First Hospital,Soochow University, Suzhou 215006, CHINA;The expression and clinical significance of PPARγ in hepatocellular carcinoma[J];Jiangsu Medical Journal;2007-11
4 LI Yan, TANG Zhaoyou, YE Shenglong, LIU Yinkun, CHEN Jie, XUE Qiong, HUANG Xiaowu, CHEN Jun, BAO Weihua, YANG Jiong, GAO Dongmei. Liver Cancer Institute and Zhong Shan Hospital of Fudan University, Shanghai, 200032, China;Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection[J];National Medical Journal of China;2002-09
5 Guo Minglei, Li Jingjun, LI Hongnian, WAN Da fang, Gu Jianren. (Department of Biochemistry , Shanghai Cancer Institute,Shanghai 200032,China);Enhanced expression of peroxisome proliferator-activated receptor γ in liver cancer[J];Tumor;2005-05
6 WU Xu-dong1,CHEN Wei-chang2,ZHANG Su-qing2(1.Department of Gastroenterology,First Affiliated Hospital of Soochow University,Suzhou 215006,China; 2.Department of General Surgery,Tumor Hospital,Nantong 226361,China);The association of expression of peroxisome proliferator-activated receptor γ with microcirculation of intra-hepatocellular carcinoma[J];Tumor;2007-11
Chinese Journal Full-text Database 10 Hits
1 Liu Hu,Ye Sheng -Long ,Li Wen-Chang ,Li Yan,Liu Yin-Kun,Qin Lun-Xiu,Qiu Shuang -Jian,Sun Rui-Xia,Chen Jie,Tang Zhao-YouLiver Cancer Institute /Zhongshan Hospital,Fudan University,Shanghai,200032,P.R.China;Quantitative Analysis of Expression of TEY1 Gene in Liver Cancer Cells with Different Metastatic Potential[J];Chinese Journal of Cancer;2003-06
2 HU Kai-wen,WEI Yue(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China);Annotation of cancer by TCM and discrimination of relevant names of diseases[J];China Journal of Traditional Chinese Medicine and Pharmacy;2011-01
3 PENG Zhihong~1, TANG Bo~2, YANG Jianmin~1, SI Suihai~1, CHEN Wensheng~1, FANG Dianchun~1, LUO Yuanhui~1 (~1Gastroenterology Research Center, ~2Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of metastasis suppressor gene KAI1 on adhesion forces of MHCC97-H hepatocellular carcinoma cells with high metastatic potential[J];Acta Academiae Medicinae Militaris Tertiae;2005-23
4 LIU Zhiyong1,OUYANG Zhong1,ZOU Xiaoming2(1. Department of Breast Surgery,the First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,Jiangxi,China;2.The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,Heilongjiang,China);Effect of Epidermal Growth Factor-like Domain 7 Gene Silencing on Angiogenesis of Stomach Carcinoma in Nude Mice[J];Journal of Guiyang Medical College;2010-03
5 Zhang Meng,He Hongtao,Zhou Ye,Xu Zhuo,Yang Tao,Liang Zhanqiang,Peng Li(Department of Hepatobiliary Surgery,Fourth Hospital,Hebei Medical University,Shijiazhuang,Hebei Province,050011,China);Expression and prognostic significance of PPAR-γ and PTEN protein in hepatocellular carcinoma[J];Journal of Third Military Medical University;2011-14
6 CHEN Liangdong,LI Yan,ZHENG Fang,LI PengchengDept.of Oncology,Zhongnan Hospital of Wuhan University & Cancer Center of Wuhan University,Wuhan 430071,China;Study on the Intraperitoneal Chemotherapy with Hydroxycamptothecin for the Treatment of Peritoneal Carcinomatosis in Mice[J];Medical Journal of Wuhan University;2008-06
7 LEI Zhan-ping,ZHU Jian-si,WANG Cheng-kun,et al(Institute of Cancer Research,Nanhua University,Hengyang,Hunan 421001,China);The Identification and Screening of the High Invasive Metastatic Strain of Mice Histiocytosarcoma L-Ⅱ[J];Journal of Nanhua University(Medical Edition);2006-03
8 NIAN Dingfang,LU lianju,ZHOU Jun,et al.Image center of Haici medical group,Qingdao,266033,China.;Clinical efficacy study of TAI/TACE combined with traditional Chinese medicine in treating advanced hepatoma[J];Chinese Imaging Journal of Integrated Traditional and Western Medicine;2005-04
9 GU Bin, WEI Xiao-ying, KUANG Sheng-jia, LI Xiao-song, ZHAO Yi-cai, ZENG Qing-e. The First Affiliated Hospital of Nanchang University, Nanchang 330006, China;Emergent transcatheter arterial embolization for ruptured liver cancer with hemorrhage[J];Journal of Interventional Radiology;2007-07
10 YANG Han-zheng,WU Xu-dong1,CHEN Wei-chang2(Institute of Immunology,Yancheng Health Vocational & Technical College,Yancheng 224006;1Department of Gastroenterology,the First People Hospital of Yancheng,Yancheng 224006;2Department of Gastroenterology,the First Affiliated Hospital of SooChow University,Suzhou 215006,China);PPARγ knockdown inhibited cell invasion and metastasis of HCCLM3 cells[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2008-07
Chinese Journal Full-text Database 4 Hits
1 WEI Na, MI Man-tian (Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China);Effects of different n-6/n-3 polyunsaturated fatty acid ratio on lipid metabolic gene expression in breast cancer cell lines[J];Acta Academiae Medicinae Militaris Tertiae;2006-07
2 CAI Tan-Xi,LIU Ping-Sheng,YANG Fu-Quan,YANG Fu-Yu(Institute of Biophysics,The Chinese Academy of Sciences,Beijing 100101,China);The Research Advances in The Field of Lipidomics[J];Progress in Biochemistry and Biophysics;2010-02
3 WEI Na, MI Man-tian, ZHANG Qian-yong, WANG Bin, YUAN Jia-lin (Department of Nutrition and Food Hygiene, Third Military Medical University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing 400038, China);EFFECT OF DIFFERENT DIETARY FATTY ACIDS ON FATTY ACID COMPOSITIONS AND LIPID METABOLIC GENE EXPRESSION IN BREAST CANCER TISSUES OF RATS[J];Acta Nutrimenta Sinica;2007-02
4 YANG Lei1, WANG Shao-kang1, SUN Gui-ju1, HU Xu2, KAI Hai-tao2, YANG Li-gang1, SU Ming2, CUI Yong-sheng2, WANG Jia-sheng3(1. Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China; 2. Center for Disease Control and Prevention of Chuzhou District, Huaian City, Huaian 223200, China; 3 The Institute of Environmental and Human Health, Texas Tech University, Lubbock TX 79409, USA);A case-control study on the risk factors for esophageal cancer[J];Tumor;2009-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HE Xiaoyan, ZHANG Min, CHEN Zhichao, YOU Yong, TIAN Lei, ZOU Ping. Department of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R.China;Expression of PPAR-γ and its apoptotic significance in lung cancer[J];Chinese Journal of Lung Cancer;2006-01
2 Kang Yi,Li Xiuling,Yang Yuxiu,Wang Tiancai 1(Department of Gastroenterology,Henan Province People 's Hospital,Zhengzhou 450003,1Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);;THE DETACTION AND SIGNIFICANCE OF PPARγ IN DIFFERENT HEPATIC DISEASES[J];Chinese Journal of Histochemistry and Cytochemistry;2006-05
3 Li Gongjie, Yang Li, Shi Xiaolin et al. 307 Hospital of PLA, Beijing 100039, China;Study of morphological characteristics of tumor microvasculature in primary hepatocellular carcinoma[J];Medical Journal of Chinese People's Liberation Army;2004-07
4 Ma Jianhua, Bai Jinliang, Fu Wu, Yang XiaochunDepartment of Urology, the First Hospital of Lanzhou University, Lanzhou 730000, China;Study on the correlation between the expression of CD146 and MVD in prostate cancer[J];Chinese Journal of Andrology;2005-04
5 SHEN Ling,ZHAO Wei,SHU Weiping,et al. Nanjing Second Hospital,Nanjing 210003, CHINA;The related factors in diagnosing early-phase hepatic cancer in young patients with hepatitis or cirrhosis[J];Jiangsu Medical Journal;2005-06
6 Xu Zhiyuan Reviwed by Wu Wanyin Department of Oncology, Guangdong Provincial Hospital of Traditional Chinese Medicine!Guangzhou;The Comparison of Different Clinical Stage of Primary Liver Cancer[J];Chinese Journal of Clinical Oncology;2005-12
7 LI Yan TANG Zhaoyou YE Shenglong,Liver Cancer Institute,Zhongshan Hospital,Fudan University,Shanghai 20032,P.R.China;Isolation and establishment of human hepatocellular carcinoma cell clones with various metastatic potentiais[J];Chinese Journal of Hepatobiliary Surgery;2001-11
8 ZHAO Xiu-lan, DU Jing, ZHANG Shi-wu, LIU Yi-xin, WANG Xin, SUN Bao-cun. Department of Pathology, Tianjin Medical University, Tianjin 300070, China;A study on vasculogenic mimicry in hepatoceliuiar carcinoma[J];Chinese Journal of Hepatology;2006-01
9 CHEN Xing guo QIAN Feng ZHANG Li Institute of Hepatology,General Hospital o.f Chinese Peoples Armed Police Forces,Beijing 100039,China;Tumor microvessel density detected by anti-CD34 as a predictor of recurrence of hepatocellular carcinoma after liver transplantation[J];Chinese Journal of Organ Transplantation;2006-10
10 ZHANG Siwei, LI Liandi, LU Fengzhu, et al. National Office of Cancer Prevention and Treatment, Beijing 100021;Mortality of primary liver cancer in China from 1990 through 1992[J];CHINESE JOURNAL OF ONCOLOGY;1999-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved