Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer Prevention and Treatment》 2012-01
Add to Favorite Get Latest Update

Inhibitory effect of siRNA targeted silencing hyrdC gene on growth of gastric cancer cell lines SGC-7901

YANG Ting-song,BI Jian-wei,SHEN Xiao-jun,WEI Guo,HUANG Sheng-dong,YUAN Yang Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,P.R.China  
OBJECTIVE:To observe the effect on growth of gastric cancer cell lines SGC-7901 induced by RNAi targeted silencing hyrdC gene.METHODS:The constructed recombinant adenovirus Ad-hyrdC mediated hyrdC gene and Ad-shyrdC knocked hyrdC gene were transfected to gastric cancer SGC-7901 cell line,the cell growth was investigated by trypan blue cell count and MTT assay in vitro,nude mice model was construted by inoculating gastric cancer cell subcutaneously into nude mice in vivo,and the tumor growth of each group was investigated.RESULTS: The cell growth of Ad-shyrdC group were significantly slower as compared to Ad-shyrdC,Ad-null and control group(P=0.02);The MTT value of Ad-shyrdC group were significantly lower as compared to Ad-hyrdC,Ad-null and control group(P=0.03);The tumor growth rate of Ad-shyrdC group was significantly lower as compared to Ad-hyrdC,Ad-null and control group(P=0.02).CONCLUSION: HyrdC gene have function of promoting the gastric cancer cell growth,the knock-out of hyrdC gene can inhibit the growth of gastric cancer cell,which can become a new promising target for gastric cancer gene therapy.
【Fund】: 国家自然科学基金(30371403)
【CateGory Index】: R735.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved