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《Chinese Journal of Cancer Prevention and Treatment》 2013-20
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Bcl-2 siRNA increase the sensitivity of gastric cancer cells to paclitaxel treatment by promoting it's apoptosis

CHEN Xi;LIN Zhen-xing;LIN Jin-juan;CHEN Ying-yu;YU Zong-yang;OUYANG Xue-nong;Department of Oncology,Nanjing Command Fuzhou General Hospital;Fujian Institute of Hematology,Union Hospital Affiliated Fujian Medical University;  
OBJECTIVE:To investigate if downregulation of Bcl-2 by siRNA would sensitize gastric carcinoma cell SGC-7901 to paclitaxel.METHODS:Bcl-2 siRNA was transfected into human gastric carcinoma cell by cationic liposome HiperFect,The expression of Bcl-2 gene mRNA and its protein in gastric carcinoma cells were evaluated by semi-quantitative RT-PCR and Western blot respectively,The cell apoptosis were examined by AnnexinⅤstaining and cell cycle assay, The chemosensitivity of transfected cell to paclitaxel was determined by MTS assy.RESULTS:Bcl-2 siRNA significantly downregulated the expression of Bcl-2 gene in human gastric carcinoma cells at both mRNA(87.6±5.3)%(P=0.001) and protein levels(85.7±3.6)%(P=0.003).The cellular apoptosis of Bcl-2 siRNA group was 66.00%,paclitaxel group was 49.00%,paclitaxel and Bcl-2 siRNA group was 46.10%.Combination of Bcl-2 siRNA with paclitaxel significantly increased the rate of apoptosis(48.00%)comparing with siRNA(9.03%)or paclitaxel(21.50%) treated alone. The IC50 of groups of Bcl-2 siRNA,negative siRNA and untreated cells siRNA were(7.25±0.22),(54.45±0.82)and(68.9±0.91)μg/L.The Bcl-2 siRNA transfected SGC-7901 cells were much more sensitive to the cytetoxic effect of paclitaxel with 86.7% decrease in the value of IC50 compared to the negative siRNA transfected SGC-7901 cells.Bcl-2 siRNA increased the sensitivity of SGC-7901 to paclitaxel by sevenfold.CONCLUSION:The effective siRNA targeting Bcl-2 can downregulat Bcl-2 gene expression and enhanc the chemosensitivity of the SGC-7901 gastric cancer cells to paclitaxel through promoting aptoposis in SGC-7901 cells,it is expected to provide a novel therapy approach for gastric cancer.
【Fund】: 福建省自然科学基金(Z0516080)
【CateGory Index】: R735.2
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