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《Journal of Qiongzhou University》 2014-05
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Isolation and Primary Identification of Actinobacteria from Rhizosphere Soil of Musa itinerans in Diaoluo Mountain

XU Xiao-xiong;LEI Min;SHAO Gui-yin;XU Yun-sheng;School of science and technology,Qiongzhou University;Key Laboratory of Tropical Crop Molecular Breeding of Sanya,Qiongzhou University;  
In order to isolate actinobacteria from rhizosphere soil of wild M. itinerans Cheesman,soil sample were collected in Diaoluo Mountain,Hainan,China. GA and HV media were employed as the isolation media that supplemented with 50 mg /L cycloheximide,50 mg /L nystatin,50 mg /L K2Cr2O7 and 0. 5 mg /L multi-vitamin. Forty-five actinobacteria were isolated according to their morphological characteristics. Nineteen isolates were putatively identified to genus level based on 16 S rRNA gene sequence analysis,which belong to Micromonospora( 7),Nonomuraea( 4),Streptomyces( 2),Verrucosispora( 4),Pseudonocardia( 1) and Nocardiopsis( 1).
【Fund】: 国家自然科学基金(21262026);; 海南省自然科学基金项目(314088);; 琼州学院青年教师科研基金项目(QYQN201430)
【CateGory Index】: S154.3
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