Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Tropical Diseases and Parasitology》 2008-01
Add to Favorite Get Latest Update

Diagnosis on HCMV infection in children by Real-time Fluorescence Quantitative PCR for HCMV-DNA detection and cell culture for virus isolation

Fang Youbing1,2,Liu Qian1,Wang Mingli1,Shen Ji-jia1.1.Department of Microbiology and Parasitology,Anhui Medical University.2.Department of Laboratory Medicine,the first Affiliated Hospital,Anhui Medical University,Hefei 230032,China.  
Objective To evaluate the application of Real-time Fluorescence Quantitative PCR for HCMV-DNA detection and cell culture for HCMV isolation on the diagnosis of HCMV infection in chil-dren.Methods The Real-time Fluorescence Quantitative PCR for detecting HCMV-DNA and the cell cul-ture technique for isolating HCMV in urine were used respectively,and the results were compared.Results ⑴ The Real-time Fluorescence Quantitative PCR for HCMV-DNA detection did not have cross reaction with related RSV.Adenovirus and HSV-1,and 20 normal children were all negative.⑵ 40 clinical cases were detected urine HCMV-DNA by the Real-time Fluorescence Quantitative PCR,and 10 cases were positive.6 cases were positive(6/11) in cleft lip(palate) group and 4 cases were positive(4/29) in pneumonia group.There was significant difference(χ2=5.06,P0.05)between the two groups.The positive rate in cleft lip(palate) group was higher than that of the pneumonia group.⑶ The 40 clinical cases were detected HCMV by virus isolation,8 cases were positive.4 cases were positive in lip(palate) split group(4/11) and 4 cases were positive in pneumonia group(4/29).There was no significant difference(χ2=1.21,P0.05) between the two groups.⑷ There was no significant difference(χ2= 3.33,P0.05) between the Real-time Fluorescence Quantitative PCR and the cell culture technique on the diagnosis of HCMV infection in children.Conclusion The Real-time Fluorescence Quantitative PCR for detecting HCMV-DNA combined with the cell culture technique for iso-lating HCMV in urine can raise the positive rate on the diagnosis of HCMV infection in children.
【CateGory Index】: R450
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved