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《Journal of Tropical Oceanography》 2012-06
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Analysis of i-type lysozyme gene from Crassostrea hongkongensis and the effect of temperature on the gene expression

XU You-qing1,WU Wei-jun1,DING Zhao-kun1,PAN Zhi-zhong2,HE Wei2,WU Tie-jun2,CHEN Xiu-li2,LI Yong-mei2,JIANG Wei-ming2 1.Institute for Fishery Sciences,Guangxi University,Nanning 530004,China;2.Guangxi Institute of Fishery Sciences,Nanning 530021,China  
Lysozyme is one of the important effectors involved in a variety of immune responses in innate immune system of organisms.A conservative i-type lysozyme cDNA sequence was cloned from Crassostrea hongkongensis and its expression in different tissues was studied.The effects of different temperatures on the gene expression in the experiment were also studied.The cloned conservative i-type lysozyme cDNA sequence contained 634 bp with a 420 bp open reading frame and the cDNA encoded 139 amino acids.A new conservative amino acid sequence,HNGGPRGC,and the typical features were found in the protein sequence of the i-type lysozyme from C.hongkongensis,including specific amino acid residue sequence,CL(E/L/R/H)C(I/M)C,a partial highly conservative sequence,SCG(P/Y)FQI,and the enzyme active site(Glu34,Asp45,Ser48,Trp61).Real-time quantitative PCR was used to assess the expression pattern of the i-type lysozyme gene in the digestive gland,muscle,gill,mantle,and labial palps of C.hongkongensis.The highest expression level of the i-type lysozyme gene was found in the digestive gland,the second in the gill,and the lowest in the muscle.A temperature stress experiment was conducted to study the expression control of the i-type lysozyme gene.The expression level was stable when the water temperature was at 20 25 ℃.A tendency to decrease expression was found at 6 or 13 ℃.The expression was maintained at a relatively high level throughout of 96 h at 27 ℃.The expression level was found the highest at 12 h and sharply declined after 48 h at 34 ℃.It is concluded that the expression of the i-type lysozyme gene was affected by temperature,which could be used to control the immune function of C.hongkongensis in response to external environment
【Fund】: 国家科技支撑计划项目(2008BAD94B03)
【CateGory Index】: S917.4
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 XU You-qing1,WU Wei-jun1,JIANG Wei-ming2,DING Zhao-kun1( 1.Institute for Fishery Sciences,Guangxi University,Nanning 530004,China;2.Guangxi Institute of Fishery Sciences,Nanning 530021,China);Effect of Temperature on Immune System and the Mechanism in Shellfish[J];Fisheries Science;2012-03
2 LIN Qun, LIANG Xu-fang, WANG Lin, LI Guang-zhao, HU Yong-le College of Life Science and Technology, Jinan University, Guangzhou 510632;Cloning and Analysis of the Beta-Actin Gene in Jinjiang Oyster (Crassostrea ariakensis) and Other Six Cultivated Aquatic Animals[J];Asian Journal of Ecotoxicology;2008-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LONG Hua (Key Opening Laboratory of Freshwater Fishery Germplasm Resources & Biotechnology,Ministry of Agriculture;Yangtze River Fishery Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000,China;Bioscience College,Wuhan University,Wuhan,Hubei 430072,China);Research Advances on 10 Nonspecific Immune Molecules of Aquatic Animals[J];Journal of Yangtze University(Natural Science Edition)Agricultural Science Volume;2005-04
2 YU Jian-feng1,2,WANG Lei2,ZHANG Yan-ping2,LI Jian-lin1,GU Zhi-liang2(1.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Science,Wuxi 214081,China;2.School of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500,China);Cloning and Expression Analysis of β-actin Gene in Elopichthys bambusa[J];Journal of Changshu Institute of Technology;2011-04
3 ZHANG Bo WANG Lin-Mei YE Bo ZHAO Zhen-Jun FAN Qi LI Shu-Ying* (Dalian Institute of Biotechnology,Liaoning Academy of Agricultural Sciences,Key Laboratory for Wild Silkworms of Liaoning Province,Dalian Liaoning 116023,China);Cloning and Sequence Analysis of Lysozyme Gene From the Chinese Oak Silkworm,Antheraea pernyi[J];Science of Sericulture;2009-03
4 YAO Cui-luan~1, WANG Zhi-yong~ 1,* , XIANG Jian-hai~2 (1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Institute of Oceanology, the Chinese Academy of Sciences, Qingdao 266071, China);Crustacean Haemocytes and Their Function in Immune Responses[J];Zoological Research;2006-05
5 PENG Huai-ming,ZHOU Shu-lin (Division of Medical and Parasitology,Wannan Medical College,Wuhu,Anhui,241000,China);Progress on Immune Function of Molluscs[J];Progress in Veterinary Medicine;2010-08
6 Yang Ru-de,Pan Li,Guo Di (Bioscience and Bioengineering College, South China University of Technology, Guangzhou 510640, China);Identification of Halophilic Lactic Acid Bacterium by Physiological and Molecular Biological Characteristics[J];Guangzhou Food Science and Technology;2005-04
7 WANG Yun 1,LONG Gao-qun 2,ZhANG Chun-lin 2,TIAN Mao 2(1.Department of Medical Biotechnology,Guiyang Medical College,Guiyang,Guizhou,550004,China;2.Department of Biology,Guiyang Medical College,Guiyang,Guizhou,550004,China);Molecular Cloing And Function Predicting of i-type Lysozyme Gene From Periplaneta Americana[J];Progress in Veterinary Medicine;2012-09
8 PAN Bao-Ping, SONG Xin, LUO Kai-Ya, GE Duan-Yang, GAO Wei-Wei (College of Life Sciences, Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University, Tianjin, 300387);EXPRESSION OF LYSOZYME GENE IN VIBRIO ANGUILLARUM-CHALLENGED CYCLINA SINENSIS[J];Oceanologia Et Limnologia Sinica;2010-06
9 ;Status of bioinformatics and its application to marine biology[J];Marine Sciences;2004-01
10 LONG Hua Key Opening Laboratory of Freshwater Fishery Germplasm Resources & Biotechnology,Ministry of Agriculture;Yangtze River Fishery Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000,China;Bioscience College,Wuhan University,Wuhan,Hubei 430072,China;Research Advances on 10 Nonspecific Immune Molecules of Aquatic Animals[J];Journal of Yangtze University(Natural Science Edition);2005-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Gang-chun1,GU Ruo-bo1,WEN Hai-bo1,HUA Dan1,NIE Chuan2(1.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agricultural, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081;2.College of Fisheries,Nanjing Agricultural University,Wuxi 214081);Effects of environmental stress on lysozyme and superoxide dismutase of Corbicula fluminea-(Müller)[J];Journal of Anhui Agricultural University;2007-01
2 SUN Ye-yong,GAO Ru-cheng,WEN Yang-min,CHEN Ning,WANG Shuai(College of Life Science,Fujian Normal University,Fuzhou 350108,China);Effects of Temperature on Hydrolase and Antioxidase Activities in Liver of Clam Hiatula chinensis and H.diphos[J];Fisheries Science;2008-10
3 LI Wen-ji,Tan Ke-fei(Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China);The Inspiration on Massive Mortality of Scallop Patinopecten yessoensi Resolved by Japan[J];Fisheries Science;2009-10
4 XU You-qing1,CAO Zhan-wang1,2,DING Zhao-kun1,GAN Xi2(1.Institute for Fishery Sciences,Guangxi University,Nanning 530004,China;2.Guangxi Institute of Fisheries,Nanning 530021,China);Effects and Prevention of High Temperature on Fishes[J];Fisheries Science;2010-04
5 DU Li1, ZHANG Wei1,2 , LU Kui1 , WANG Feng-yang1, WANG Ai-min3(1.College of Agriculture,Hainan University,Haikou,Hainan,570228,China;2.College of Life Science ,Huazhong Normal University,Wuhan,Hubei,430079,China;3.College of Oceannology,Hainan University,Haikou,Hainan,570228,China);Progress on Immunological Mechanism in Mollusks[J];Progress in Veterinary Medicine;2008-03
6 PENG Huai-ming,ZHOU Shu-lin (Division of Medical and Parasitology,Wannan Medical College,Wuhu,Anhui,241000,China);Progress on Immune Function of Molluscs[J];Progress in Veterinary Medicine;2010-08
7 FEI Laihua1,SHAO Yanqun2,LI Yun1*(1.The Key Laboratory of Mariculture of Ministry Education,Ocean University of China,Qingdao 266003,China;2.Oceanic and fishery bureau of Chengyang district,Qingdao 266109,China);COMPARITIVE ANALYSIS OF THE ACTIVITY OF SOD,CAT AND MPO IN DIFFERENT TISSUES OF THE SCALLOP CHLAMYS FARRERI[J];Transactions of Oceanology and Limnology;2008-03
8 GAO Fei,YANG Hongsheng,XU Qiang,WANG Fangyu,LIU Guangbin Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka),with special reference to aestivation[J];中国海洋湖沼学报(英文版);2009-04
9 ;PHAGOCYTOSIS AND NON-SELF RECOGNITION OF MOLLUSCAN BLOOD CELLS:A REVIEW[J];Marine Sciences;2003-02
10 ;Advances in the studies on immunity of bivalves[J];Marine Sciences;2006-09
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