Study on pollen germination and pollen tube growth of Calotropis gigantea
BIN Zhenlan;TANG Danfeng;ZHOU Ruiyang;College of agriculture, Guangxi University;
Pollen germination and pollen tube growth of Calotropis gigantea was tested by using One-Way ANOVA, multiple comparison, an orthogonal experiment and range analysis The results indicated that the pollen germination and pollen tube growth would be enhanced when the concentration of borate, calcium nitrate, magnesium sulphate and sucrose of the culture medium were within a certain range. When the concentration is too high or low, the pollen germination and pollen tube growth would be inhibited.Potassium has little impact on pollen tube growth and pollen germination. Sucrose and calcium nitrate have significant effect on pollen germination, sucrose and magnesium sulphate have significant effect on pollen tube growth. The optimum culture medium and environment conditions which support the germination and growth are 15% sucrose +350 mg/L Ca(NO_3)2·4H_2O+60 mg/L H_3BO_3+150 mg/L Mg SO_4·7H_2O. The pollen germination and the growth of pollen tube will be better under 30~35℃.
【CateGory Index】： S567.19