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《China Tropical Medicine》 2008-05
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Extraction and analysis of lectin in 14 vegitables and its biological activity

WANG Qing-zhong1, ZHANG Lu2, WU Yun-hong2,et al. (1 .Shanghai Municipal Clinical Central Laboratory, Shanghai 200126; 2. School of Life Science and Engineering of Qiqihaer University, Qiqihaer 161000, Heilongjiang , P. R.China)  
Objective To select lectins from 14 kinds of vegetable and analyze their biological activities. Methods Crude proteins were extracted after ammonium sulfate precipitation and dialysis. Their agglutination activities were screened with erythrocytes. The bioactivities were compared by serials of tests including heat-tolerance, pH-tolerance, saccharide-repression, metalion-dependence and bacteriostasis experiments. Results The agglutination activities of the lectins cvaried in diferent vegetables andf tissues of plants. The agglutination activity of A.ledebourianum was the strongest. Optimal activity was detected under alkaline situation. Different lectins showed varied activities of bacteriostasis. Conclusion This results has set foundation for conducting development of lectins and study on its molecular biological.
【Fund】: 黑龙江省齐齐哈尔市科技局 黑龙江省教育厅资助 项目编号:10551328
【CateGory Index】: S63
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