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《Journal of Tropical Medicine》 2004-03
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Identification and Expression of the HBV Particle Antigen in Transgenic Tomato Plants

LIU Gao feng, DONG Wen qi,CHEN Li shan,LI Feng mei, REN Da ming,LI Ming,HUANG Jian sheng (The First Military Medical University,Guangzhou 510515,China)  
Objective To establish recombinant tomato for HBV particle antigen.Method Hepatitis B virus preS/S gene was inserted into the 73~94aa of the HBc(tine of the spinous tuber). Then the whole combined gene was cloned into plant expression vector pBIN438 which is under the control of the CaMV 35s promotor. Tomato was transformed by co cultivating leaf discs with Agrobacterum strains harboring pBIN438 HBc HBs.Result The regenerated kanamycin resistant transforms were analyzed by PCR ,PCR Southern blot,Southern blot and Western blot. Conclusion The results indicated that HBc HBs was integrated into the genomic DNA of the tomato, and was efficiently expressed in the tomato.
【Fund】: 国家自然科学基金项目(No.30170532);; 广州市重点攻关项目(No.2001-Z-035-01-2)。
【CateGory Index】: R346
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