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《Journal of Tropical Medicine》 2005-02
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Effect of Platelet-Derived Growth Factor-BB and Transforming Growth Factor-β on the Proliferation of Human Periodontal Ligament Cells in Vitro

JI Jian-xin,LIAO Wei-jiao,QIU Zhi-hui,PAN Guang-ci,ZHANG Ting(Department of Stomatology,First Af filiated Hospital,Guangzhou Medic al College,Guangzhou 510120,China)  
Objective To evaluate the biological effects i n vitro of platelet-derived growth f actor-BB(PDGF-BB)and /or transforming growth factor-β(TGF-β)on the proliferation in human period ontal ligament (PDL)cells.Methods PDL cells were obtained from healthy donors.Cells(6×10 5 /200μl)were cultured for 1to 5days in DMEM supplemented with 1%FBS alone(control),or containing PDGF-BB(1,5,10or 50ng /ml ),TGF-β(1,5,10or 50ng /ml )or both.Proliferation of the PDL cells was measured by MTT colorimeteric assay.Data was analyzed by ANOVA.Results PDGF-BB or TGF-βat concentration of 1to 50ng /ml show ed a significant effects.A signific ant enhanced of proliferation was seen when PDL cells were treated with 1~50ng /ml PDGF-B B or TGF-βin a dose-and time dependent manner(P0.05or P0.01).The most optimal concentration of PDGF-BB and TGF-βwas 10ng /ml and 5ng /ml,cultured for 3days and 4days,respectively.The effect of combination of PDGF-BB with TGF-βwas the most signification one(P0.001).Conclusions PDGF-BB and /or TGF-βcan promote the proliferation of PDL cells.The data suggest that these growth factors may be valu able in promoting the regeneration o f periodontal connective tissue.
【Fund】: 广州市科技局资助项目(No.1999-J-008-01)。
【CateGory Index】: R96
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