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《Chinese Journal of Tropical Crops》 1990-02
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EFFECTS OF ABA ON REGULATION OF RESISTANCE OF COFFEE SEEDLINGS TO CHILLING INJURY

Cai Shiying Zhou Qianping Pan Qiuhong (Tropical Crops Department,SCCTC)  
The effects of ABA on regulation of resistance of coffee seedlings to chillinginjury were tested in terms of the influence of the activity of enzyme system(Su-peroxide dismutase and peroxidase)and the content of nonenzyme system(Vitaman C)on the integrity of membrance structure and the enhancement of resistance to chillinginjury(electron-conductivity or relative electron-conductivity)after treatments.Thebest effects of ABA were seen in pretreatment for 24 hr at the concentration of 100-150 ppm.Remarkable effects were observed on treated leaves(or cotyledons)otherthan roots.Both the methods of leaf spraying and root soaking were able to increasethe resistance of coffee seedlings to chilling injury.Comparison studies showed that,ABA apparently increased the resistance of cof-fee seedlings to chilling injury,while IAA,BA and ethrel didn't give the effect,and ethrel even aggravated chilling injury.The action of ABA may be related to itsinvolving in the metabolic process of water loss and membrance structure integrityprotection.Under low temperature colchicine exacerbated the chilling injury.ABA how-ever,could counteract the effect of colchicine to some extent,which was most notice-able when the application of ABA was made before colchicine and low temperaturetreatments.This indicated that,the disassembly of the microtubules induced by lowtemperature was one of the factors responsible for chilling injury.It thus seems thatABA also played some part in maintaining the physiological stability of the micro-tubule network.
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