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Cytological and Biochemical Identification of Major Storage Proteins in Hevea brasiliensis Seed

Tian Weimin; Wu Jilin; Hao Bingzhong(College of Agronomy, South China University of Tropical Agriculture,Danzhou, Hainan 571737; Ministry of Agriculture Key Physiology Laboratory for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Danzh  
The embryo and prosembryum of the mature seed of Hevea brasiliensis contain about 15 % proteins and 0.6 % free amino acids based on dry weight. Eleven extractants for proteins of these tissues were tested and more than 80 % proteins were extracted by the extractant of Tris(41 mmol/L)-Bori acid (40 mmol/L) buffer (pH 8.6) with 2 % SDS. There were two groups of prominent proteins in the polypeptide profiles based on SDS-PAGE. Each group consisted of three distinguishable peptides: 36, 34 and 32 kDa peptides for group I and 20, 19 and 18.5 kDa peptides for group Ⅱ. The two groups of proteins were found mainly in the prosembryum and cotyledon of the seed. During seed development, these proteins emerged and accumulated in a great quantity, which was roughly synchronized with the emergence and increase of the vacuole protein masses in prosembryum and cotyledon cells observed under light and electron microscope. After germination, the proteins diminished continuously, and the vacuole protein masses decreased gradually as well. The results show that Group I and Ⅱ proteins are the major stomp proteins in Hevea seeds and may be the major composition of the vacuole protein masses. The recalcitrant characteristics of Hevea seeds and the differences between the seed storage proteins and the vegetative storage protein in Hevea brasiliensis were discussed.
【Fund】: 国家自然科学基金!39860066
【CateGory Index】: S794.1
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