Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Tropical Crops》 2005-02
Add to Favorite Get Latest Update

Isolation of cDNA Related to Salt-tolerance in Mangrove Clerodendrum inerme by mRNA Differential Display

Shan Jialin1,2 Zheng xueqin2 (1 College of Agronomy, SCUTA, Danzhou, Hainan 571737)2 National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101)  
The 1st strand of cDNA was synthesized from total RNA isolated from the leaves of Clerodendrum inerme respectively under fresh water and saline water condition, which was used as template for polymerase chain reaction with anchored primer oligo(dT)12CA and 10-oligonucleotide arbitrary primer. Three stable differential cDNA fragments, colb1,colb2 and colb3, were found present only in the leaves of C. inerme culturedunder the saline condition. These fragments were detected by RNA hybridization, but only fragment colb1 was identified to be a salt-tolerant cDNA, which was then cloned and sequenced. After searching BLASTN and BLASTX on the NCBI, no reported similar sequence was found.
【CateGory Index】: S718.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Shan Jialin1, Zheng Xueqin2 1 College of Horticulture and Landscape Architecture, Hainan University, Danzhou, Hainan 571737 2 State Key Biotechnology Laboratory for Tropical Crops, Chinese Academy of Tropical Agriculture, Haikou, Hainan 571101;Histochemical Features and Salt-adaptation Significance of Juvenile Absorptive Roots of Mangrove Plants[J];Chinese Journal of Tropical Crops;2009-04
【Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHOU Han-Tao\+*\ LIN Peng (School of Life Sciences,Xiamen University, Xiamen\ 361005, China);Extraction of the Salt-tolerant cDNA in Mangrove Avicennia marina by mRNA Differential Display[J];Chinese Journal of Biotechnology;2002-01
2 Lin Qifeng, Deng Yongchuan, Wu Duogui, Chen Jupei, Li Guanyi( Institute of Biological Science and Technology, Hainan University, Haikou,570228);Molecular Breeding of Salt┐ Resistant Capsicum annuum[J];PROGRESS IN BIOTECHNOLOGY;1999-05
3 Lin Qi\|feng\ Deng Yong\|chuan\ Huang Wei\ Chen Ju\|pei\ Li Guan\|yi (Institute of Biological Science and Technology,Hainan University,Haikou\ 570228);Introducing total DNA of Rhizothora apicularta to Solanum melongena to generate Salt-tolerant progenies[J];Progress In Biotechnology;2001-05
4 CUI Kairong XING Gengsheng QIN Lin WANG Yafu (The State Key Laboratory of Arid Agroecology of Lanzhou University,Lanzhou 730000);Differential Expression of the Gene in Early Somatic Embryogenesis of Lycium barbarum L by modified mRNA Differential Display[J];HEREDITAS(BEIJING);1998-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Yu-xiao1,ZHUANG Wen-chang1,WANG Jing2(1.College of Chemistry and Chemical Engineering,Xuzhou Institute of Technology,Xuzhou 221008,China;2.College of Chemical Engineering,China University of Petroleum,Beijing 102249,China);Identification through 16S rDNA Sequence Analysis and Marking with Green Fluorescent Protein of an Isolated Strain for Microbial Enhanced Oil Recovery[J];Journal of Xuzhou Institute of Technology(Natural Sciences Edition);2011-04
2 CUI Liu-fu YANG Wen-hao SONG Hai-cheng SHU Rong HAN Yi-xuan LIU Yan-ying LU Yan-ping YU Ping (Department of Rheumatology and Immunology,the Affiliated Kailuan Hospital of North China Coal Medical University,Tangshan,Hebei 063000,China);Association of Polymorphism of Peptidylarginine Deiminase 4 Gene and Rheumatoid Arthritis in Han Population[J];Chinese Journal of Allergy & Clinical Immunology;2007-02
3 LI Chun-juan~1, WAN Shu-bo~1, XU Ting-ting~1, ZHUANG Wei-jian~2 , SHAN Shi-hua~1(1.Shandong Peanut Research Institute, Qingdao 266100, China; 2.Crop Scientific College of Fujian A&F University, Fuzhou 350002, China);Research of Influence Factors on Peanut Transformation Mediated by A. tumefaciens[J];Journal of Peanut Science;2004-04
4 ZHANG Hong-tao~(1,2),SHAN Lei~2,QUAN Xian-qing~(1,2,3),BI Yu-ping~(1,2),YANG Jia-sen~(1,2),WANG Xiu-li~2(1.College of Life Sciences,Shandong Normal University,Ji'nan 250014,China;2.High-Tech Research Center,Shandong Academy of Agricultural Sciences,Ji'nan 250100,China;3.Linyi Normal University,Linyi 276000,China);Functional Analysis of an Arachis hypogaea L.△12 Fatty Acid Desaturase Gene by Heterologous Expression in Saccharomyces cerevisiae[J];Journal of Peanut Science;2006-01
5 LIANG Xue-lian~1,ZHENG Yi-xiong~1,ZHUANG Dong-hong~2,HUANG De-qiang~1(1.College of Life Sciences,Zhongkai University of Agricultural and Technomogy,Guangzhou 510225,China;2.College of Life Sciences,Shantou University,Shantou 515063,China);Peanut Anti-pest Gene Transformation by Pollen-mediation[J];Journal of Peanut Science;2006-04
6 YU Shan-lin,CHI Xiao-yuan,YANG Qing-li,PAN Li-juan,HE Ya-nan,CHEN Ming-na,YANG Zhen(Shandong Peanut Research Institute, Qingdao 266100, China);Construction of a Full-length cDNA Library from Peanut Seedlings[J];Journal of Peanut Science;2010-02
7 WANG Hong-liang(Department of Mathematics,Xinxiang University,Xinxiang 453003,China);Cloning of Mouse Liver-expressed Antimicrobial Peptide -2 gene and Construction of the Expression Vector[J];Journal of Xinxiang University(Natural Science Edition);2010-02
8 GE Xiao-ping SHI Yan-jing (College of Chemical Engineering,Qingdao University of Science and TechnOlogy,Qingdao 266042,China);A Useful Method for Extracting RNA from Plant Tissues Rich in Polysaccharides and Polyphenol[J];Journal of Qingdao University of Science and Technology(Natural Science Edition);2007-01
9 CHEN Xiao~1, JIANG Han-Dong~2, LU Xin-Zhi~1, YU Wen-Gong~1(1. Key Laboratory of Marine Drugs,Ministry of Education, Key Laboratory of Marine Drugs of Shangdong Province, Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, China; 2. Respiratory Department of the Affiliated Hospital, Medical College of Qingdao University, Qingdao 266003, China);Establishment of a High-Efficiency Calcium Phosphate Transfection Method for HEK 293T Cells[J];Journal of Ocean University of Qingdao;2005-05
10 ZHOU Yi-Jiang,ZHANG Xue-Cheng,WANG Yu-Mei,ZANG Xiao-Nan(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China);The Tandem Expression of the Human/Salmon Chimeric CT Gene in E.coli[J];Periodical of Ocean University of China;2007-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Sun Shaoxian (South China Sea Institute of Oceanology, Academia Sinica, Guangzhou 510301);DISTRIBUTION OF SONNERATIA POLLEN IN THE LATE QUATERNARY STRATA OF THE SOUTH CHINA SEA AND ITS SIGNIFICANCE[J];Tropic Oceanology;1991-01
2 LI Baocai1,2,3,WEN Kewei2 ,DONG Yulian1, ZHOU Jianyuan2 , SHENG Guoyin1,FU Jiamo1ochemistry, The Chinese Academy of Sciences; hou 510640, China;2. Application Technique Lab of Marine Organisms, South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou 510301, China; 3. College of Biological and Chemical Engineering, Kunming University of Technology, Kunming 650051, China );SEPARATION AND IDENTIFICATION OF RUTIN IN LEAVES OF KANDELIA CANDEL FROM DANZHOU, HAINAN PROVINCE[J];Tropic Oceanology;2002-03
3 HUANG Fu, SONG Wen-dong, JIA Zhen-yu(Department of Applied Chemistry, Zhanjiang University of Ocean, Zhanjiang 524006,China);CHARACTERISTICS OF CHEMICAL CONSTITUENTS OF VOLATILE OIL FROM LEAVES OF MANGROVE PLANT KANDELIA CANDEL BY GAS CHROMATOGRAPHY/MASS SPECTROMETRY[J];Journal of Tropical Oceanography;2005-04
4 YANG Zhen,DENG Ye-cheng,CHEN Xin-hua(College of Life Science,Guangxi Normal University,Guilin 541004,China);Antifungal and antibacterial activities of methanol extracts from mangrove plants[J];Journal of Tropical Oceanography;2007-01
5 Shan Jia-lin 1、2 ,Zheng Xue-qin 2 (1.South China University of Tropical Agriculture.Dan Zhou Hainan571737;2.National Key Biotechnology Laboˉratory for Tropical Crops,CATAS,Haikou571101);Preliminary Studies on Flora of Mangrove Forest in Hainan Island[J];Tropical Forestry;2005-03
6 Wang Guofen Dai Peng Peng Jun Xie Yixian Huang JunshengHainan Key Laboratory for Tropical Agricultural Pest Detection and Supervision/Environment and Plant Protection Institute Danzhou Hainan 571737;Identification and ITS Sequence Analysis of the Pathogens of Banana Sigatoka Disease[J];Chinese Journal of Tropical Crops;2006-04
7 Xi Jingen Sun Guangming Zang Xiaoping He Yingdui South Subtropical Crops Research Institute, CATAS Zhanjiang Guangdong 524091;Effects of Zinc Levels on Growth and Development and Physiological Metabolism in Pineapple[J];Chinese Journal of Tropical Crops;2007-04
8 Wang Ruijiang; Chen Zhongyi; Huang Xiangxu (South China Institute of Botany, Academia Sinica. Guangzhou 510650);CHROMOSOME COUNTS ON CHINESE MANGROVES[J];JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY;1998-01
9 Li Dehong; Wang Xiaojing; Pan Ruichi(Department of Biology , South China Normal University, Guangzhou 510631);CHANGES IN CONTENT OF CALMODULIN IN IAA-AND 6-BA-INDUCED SWELLING OF PROTOPLASTS FROM HYPOCOTYL IN ETIOLATED MUNG BEAN SEEDLINGS[J];JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY;1999-03
10 QIU Dong-lang LIN Peng(School of Life Science, Xiamen University, Xiamen 361005, China);Advances in Molecular Mechanisms of Salt Tolerance in Plants[J];Journal of Tropical and Subtropical Botany;2002-03
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 JIA Jin1,2 and ZHANG Lu-gang1(1 College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;2School of Biological & Chemical Engineering,Inner Mongolia University of Science & Technology,Baotou,Inner Mongolia 014010,China);Cloning and Sequence Analyzing of Malate Dehydrogenase Gene in Chinese Cabbage [Brassica campestris L.ssp.pekinensis (Lour.) Olsson][J];Acta Horticulturae Sinica;2009-03
2 ZHANG Li-li1,2,LIU Yu-mei2,TIAN Zi-hua1,FANG Zhi-yuan2,YANG Li-mei2,ZHUANG Mu2,ZHANG Yang-yong2,LI Zhan-sheng2,and SHU Jin-shuai2(1College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010018,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Advances of Research on Breeding of Resistance to Black Rot in Crucifer[J];Acta Horticulturae Sinica;2012-09
3 LI Tie-jun;LIAN Mei-lan;SHAO Chun-hui;YU Dan;PIAO Xuan-chun;Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular Yanbian University;;Effects of acid and alkali stress on ginsenoside content and histochemical localization of ginsenoside in adventitious root of Panax ginseng[J];China Journal of Chinese Materia Medica;2013-24
4 LIU Li;College of Agriculture,Eastern Liaoning University;;Similarity of the Floristic Characteristics of Different Coastal Wetlands in China[J];Plant Science Journal;2014-05
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Liang Zheng 1\ Ma Deqin 2\ Tang Lan 1\ Hong Yiguo 2\ Luo Ailing 1\ Dai Xiuyu 2 (Institute of Botany,Academia Sinica,Beijing 100093) 1 (Institute of Microbiology,Academia Sinica,Beijing 100080) 2;Expression of the Spinach Betaine Aldehyde Dehydrogenase (BADH) Gene in Transgenic Tobacco Plants[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1997-03
2 Zhang Hui Dong Wei Du Baoxing Zhou Junma Gu Dongmei Chen Shouyi (Laboratory of Plant Biotechnology,Institute of Genetics,The Chinese Academy of Sciences,Beijing 100101)Chinese Center,DeMontfort University,Norman Borlaug Center for Plant Science,C;The Cloning of Levansucrase Gene and Its Engineering of Salttolerant Tobacco Plants[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1998-02
3 Lin Qifeng, Deng Yongchuan, Wu Duogui, Chen Jupei, Li Guanyi( Institute of Biological Science and Technology, Hainan University, Haikou,570228);Molecular Breeding of Salt┐ Resistant Capsicum annuum[J];PROGRESS IN BIOTECHNOLOGY;1999-05
4 WU Duogui,LIN Qifeng,LI Guanyi (Institute of Biological Science and Technology,Hainan University,Haikou 570228);Cetyl trimethyl Ammonium Bromide(CTAB)Method for Extracting Rhizophora apiculata's DNA and its Random Amplified Polymorphic DNA(RAPD)Reaction[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-01
5 LIU Fenghua GUO Yan GU Dongmei XIAO GangCHEN Zhen hua CHEN ShouyiAuthor for correspondence(Institute of Genetics,Chinese Academy of Science Beijing 100101);Salt Tolerance of Transgenic Plants With BADH cDNA[J];ACTA GENETICA SINICA;1997-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved