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《Chinese Journal of Tropical Crops》 2005-02
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Isolation of cDNA Related to Salt-tolerance in Mangrove Clerodendrum inerme by mRNA Differential Display

Shan Jialin1,2 Zheng xueqin2 (1 College of Agronomy, SCUTA, Danzhou, Hainan 571737)2 National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101)  
The 1st strand of cDNA was synthesized from total RNA isolated from the leaves of Clerodendrum inerme respectively under fresh water and saline water condition, which was used as template for polymerase chain reaction with anchored primer oligo(dT)12CA and 10-oligonucleotide arbitrary primer. Three stable differential cDNA fragments, colb1,colb2 and colb3, were found present only in the leaves of C. inerme culturedunder the saline condition. These fragments were detected by RNA hybridization, but only fragment colb1 was identified to be a salt-tolerant cDNA, which was then cloned and sequenced. After searching BLASTN and BLASTX on the NCBI, no reported similar sequence was found.
【CateGory Index】: S718.4
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1 Shan Jialin1, Zheng Xueqin2 1 College of Horticulture and Landscape Architecture, Hainan University, Danzhou, Hainan 571737 2 State Key Biotechnology Laboratory for Tropical Crops, Chinese Academy of Tropical Agriculture, Haikou, Hainan 571101;Histochemical Features and Salt-adaptation Significance of Juvenile Absorptive Roots of Mangrove Plants[J];Chinese Journal of Tropical Crops;2009-04
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