Establishment of a Disease-free Seed Canes Propagation System for Sugarcane
Yang Benpeng Zhang Shuzhen Yang Xue Wang Yeqiao Gu Lihong Feng Cuilian Cai Wenwei Zhang Xianyong State Key Biotechnology Laboratory of Tropical Crops/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences (CATAS) Haikou 571101 China
Disease-free seed canes are free from known sugarcane diseases(Saccharum L.) specially ratoon stunting disease (RSD) and sugarcane mosaic virus (SCMV). Application of disease-free seed canes as planting materials in commercial production of sugarcane ensures high yield and high quality of sugarcane. An efficient technical system for producing disease-free seed canes of sugarcane involving combination of hot water treatment (HWT) and thermotherapy with apical meristem culture was developed. The axillary buds germinated from the canes, which were treated by HWT and followed by the thermotherapy, were used as explants. The apical meristems of axillary buds were inoculated on the MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 200 mg/L PVP and 30 g/L sucrose for inducing shoots. After having been cultured for 10-20 days, the shoots bourgeoned. They were then transferred onto the MS medium supplemented with 1 mg/L BA , 0.5 mg/L KT and 30 g/L sucrose for inducing cluster buds. After 15~20 days, the cluster buds were formed. The proliferation ratio of the cluster buds is about 3~5 buds per 15~20 days. The cluster buds were divided into single buds and cultured on the 1/2MS medium containing 1.0 mg/L NAA and 20 g/L sucrose for inducing roots. After 10~20 days, the roots were formed and transferred onto the soil. About 98 % of plantlets survived when they were transferred onto the soil. The plantlets grew well and uniform without any variation. The results of molecular detection demonstrated that the plantlets were free from RSD and SCMV. This system will be a good foundation for commercial production of disease-free seed canes of sugarcane.