Assessment of Genetic Diversity of 12 Signal Grass Germplasms by ISSR Markers
Chen Xiaobin1,2 Zou Dongmei1 Tian Weimin1 Jiang Changshun1 1 Key Open Laboratory for Cultivation and Physiology of Tropical Crops, CATAS Danzhou Hainan 571737 2 College of Agronomy, Hainan University Danzhou Hainan 571737
Genetic diversity of 12 accessions of signal grass(Brachiara Griseb) was evaluated by using 17 ISSR primers with inter-simple sequence repeat(ISSR) markers. The results showed that high genetic diversity in the signal grass germplasms was detected. A total of 286 bands were amplified from 17 primers, of which 280 bands (97.90 %) were polymorphic, and 6 to 23 polymorphic bands could be amplified from each primer, with an average of 16.8. The mean genetic similarity value based on ISSR markers among 12 accessions of signal grass was 0.681, ranging from 0.486 to 0.968. Cluster analysis showed 12 accessions of signal grass could be classified into 2 groups. One group included B. mutica(Forssk.) Stapf and B. humidicola(Rendle) Schweick, and the rest of the accessions were clustered into the other group.