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《Chinese Journal of Tropical Crops》 2009-11
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Analysis of Genetic Diversity of Tea Plants by Using EST-SSR and ISSR Markers

Liu Benying1,2,Sun Xuemei1,Li Youyong1,Tang Yichun1,He Wei2,Wang Yungang1,Wang Pingsheng1 1 Tea Research Institute,Yunnan Academy of Agricultural Science,Menghai,Yunnan 666201;2 State Center for Tea Improvement;Research Center for Tea Germplasm Resources and Genetic Improvement,Tea Research Institute,Chinese Academy of Agricultural Science,Hangzhou 310008  
Two different DNA-based techniques,inter-simple sequence repeats(ISSR) and(expressed sequence tags) ESTs-derived SSRs(simple sequence repeats),were used for detecting genetic diversity of 33 accessions of tea germplasm.Using 12 ISSR primers,248 discernible DNA fragments were generated with 228(91.94%) being polymorphic,and the mean PIC value of each ISSR primer was 0.318.Thirty EST-SSR primers produced an average of 4.9 alleles/locus and an average PIC value of 0.499.Both DNA-based techniques were able to amplify all of the genotypes,showing a high genetic diversity among tea germplasm resources tested.ISSRs and SSRs were compared in terms of their informativeness and efficiency in analysis of genetic diversity and relationships among 33 tea accessions,and ISSRs presented a greater informative content.The mean genetic similarity coefficients among all tea accessions ascribed by ISSR and EST-SSR matrices were close,0.709 and 0.743,respectively.Some differences in clustering results were observed.The correlation coefficients of similarity were statistically significant for both marker systems used(r=0.817,P﹤0.01).It could be inferred that these 2 molecular fingerprinting techniques were both favorable for study of tea genetic diversity.
【Fund】: 国家科技基础条件平台建设计划项目(No.2005DKA21002);; 浙江省重点科技计划项目(No.2006C22070)资助
【CateGory Index】: S571.1
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