Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Guangzhou University of Traditional Chinese Medicine》 2003-03
Add to Favorite Get Latest Update

In-Vitro Induction of Mesenchymal Stem Cells Differentiation intoNeurons in Adult Rats by Serum ContainingCarapax et Plastrum Testudinis

CHEN Dongfeng, DU Shaohui, LI Yhuei, LI Hui ( Dept. of Anatomy, Guangzhou University of TCM, Guangzhou 510405, China; Dept. of Internal Medicine, Shenzhen Hospital of TCM, Shenzhen, China)  
[Objective] To observe the effect of serum containing Carapax et Plastrum Testudinis (CPT) in inducing the differentiation of mesenchymal stem cells (MSC) of adult rats into neurons. [Methods] The fifth generation of MSC were induced by the serum containing CPT (sero-CPT) and the expressions of neurofilament (NF) protein and glial fibrillary acidic protein (GFAP), the marks of neuron-like cells were detected by immunohistochemical method. [Results] The expression of NF protein was positive after the stimulation of sero-CPT and the expression reached the peak 12 hours after induction. [Conclusion] Sero-CPT can induce the differentiation of MSC into neurons in vitro.
【Key Words】: CARAPAX ET PLASTRUM TESTUDINIS/phaimacology BONE MARROW CELLS/ ultrastructure STROMAL CELLS/ ultrastructure NEURONS/ultrastructure CELL DIVISION/drug effects
【Fund】: 国家自然科学基金项目(编号:30271677)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yue-hua et al (Guangzhou City Polytechnic,Guangzhou,Guangdong 510405);Research and Application of GC-MS Stem Cell Activity Fingerprint of Methyl-esterified Tortoise Shell Extract[J];Journal of Anhui Agricultural Sciences;2008-01
2 Zhang Yue-Hua1,2,Zeng He-Ping1,Chen Dong-Feng3 1(Institute of the Functional Molecule,Department of Chemistry,South China University of Technology,Guangzhou 510641) 2(Guangzhou City Polytechnic,Guangzhou 510405) 3(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405);Effect of Fatty Acids Methyl Ester from Plastrum Testudinis on Proliferation of Rat Bone Mesenchymal Stem Cell[J];Chinese Journal of Analytical Chemistry;2007-10
3 WANG Chun-yan,ZENG He-ping,CHEN Wei(School of Chemistry & Environment,South China Normal University Guangzhou 510631,China);EFFECTS ON PROLIFERATION OF RAT MESENCHYMAL STEM CELL AND CHEMICAL COMPONENTS FROM PLASTRUM TESTUDINIS OBTAINED BY GC-MS[J];Journal of South China Normal University(Natural Science Edition);2007-01
4 HU Xiang-yang1,ZHANG Rong-hua2(1.Doctoral Student of Grade 2005,Medical College of Jinan University,Guangzhou 510632,Guangdong,China;2.Pharmacy College of Jinan University,Guangzhou 510632,Guangdong,China);Advances in Differentiation of Bone Mesenchymal Stem Cells in Rats Into Neuron-like Cells Inducing by Chinese Herbal Medicine[J];Liaoning Journal of Traditional Chinese Medicine;2007-11
5 ZHENG Jie, WANG Peixun, ZHOU Lian(Institute of Immunology,Guangzhou University of TCM,Guangzhou 510405,China);Isolation of Rat Marrow Mesenchymal Stem Cells and Effect of Conditioned Medium on Its Proliferation[J];Journal of Guangzhou University of Traditional Chinese Medicine;2006-04
6 ZHANG Yue-Hua~1, ZENG He-Ping~ 2 and CHEN Dong-Feng~31 Guangzhou City Polytechnic College, Guangzhou 510405, China2 Institute of the Functional Molecule, Department of Chemistry, South China University of Technology, Guangzhou 510641, China3 Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China;Effect of Fatty Acids from Plastrum Testudinis on Proliferation of Rat Bone Mesenchymal Stem Cell[J];Chinese Journal of Biotechnology;2007-06
7 Chen Dongfeng1, Li Hui1, Du Shaohui2, Zhou Jianhong1, Li Yiwei1, Zhang Liqun1, Deng Rudong1, Zhang Saixia1 (1Department of Anatomy, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405; 2Department of Internal Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518000);EFFECTS OF PLASTRUM TESTUDINIS ON DIFFERENTLATION OF TRANSPLANTED MESENCHYMAL STEM CELLS INTO NEURONS IN THE INJURED SPINAL CORD OF RAT[J];Chinese Journal of Neuroanatomy;2006-02
8 ZHANG Yue-hua1,ZENG He-ping2,CHEN Dong-feng3(1.Guangzhou City Polytechnic College,Guangdong Guangzhou,510405 China;2.Institute of the Functional Molecule,Department of Chemistry,South China University of Technology,Guangdong,Guangzhou,510641 China;3.Guangzhou University of Traditional Chinese Medicine,Guangdong Guangzhou 510405,China);The Analysis and Application of Plastrum Testudinis GC-MS Fingerprint that Promotes the Proliferation of Stem Cells[J];Lishizhen Medicine and Materia Medica Research;2008-05
9 WANG Nan-ding;LI Zhe;ZHANG Xiao-ping;WANG Zong-ren;Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University;Youth League Committee, Shaanxi University of Chinese Medicine;Humanistic science, Shaanxi University of Chinese Medicine;;The Features and Progress of Traditional Chinese Medicine in Stem Cell Research[J];Progress in Modern Biomedicine;2013-36
10 Wang Qian;Fan Wentao;He Youshun;Hunan University of Chinese Medicine;;Effect of Dihuang Yinzi protein on hippocampal slices of SDF-1in rats[J];Shaanxi Journal of Traditional Chinese Medicine;2015-03
China Proceedings of conference Full-text Database 1 Hits
1 ;The experimental study about the effect of Sheng-Ji liquor on proliferation and osteogenesis of bone mesenchymal stem cells[A];[C];2006
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Dongfeng 1, DU Shaohui 2, LI Yiwei 1, ZHANG Jin 1, LI Hui 1 (1.Dept.of Anatomy,Guangzhou University of TCM,Guangzhou 510405,China; 2.Shenzhen Hospital of TCM,Shenzhen 518033,China);Effect of Plastrum Testudinis on Neural Stem Cell After Focal Cerebral Ischemia[J];Journal of Guangzhou University of Traditional Chinese Medicine;2001-04
2 CHEN Dongfeng1,DU Shaohui2,LI Yiwei1,ZHANG Jin1,LI Hui1(1.Department of Anatomy, Guangzhou University of TCM, Guangzhou 510405; 2.Shenzhen Hospital of TCM,Guangdong Shenzhen 518033);Effect of Plastrum Testudinis on Three Subtypes of NOS Expression in Rats with Focal Cerebral Ischemia[J];Traditional Chinese Drug Research & Clinical Pharmacology;2002-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yue-hua et al (Guangzhou City Polytechnic,Guangzhou,Guangdong 510405);Research and Application of GC-MS Stem Cell Activity Fingerprint of Methyl-esterified Tortoise Shell Extract[J];Journal of Anhui Agricultural Sciences;2008-01
2 Zhang Yue-Hua1,2,Zeng He-Ping1,Chen Dong-Feng3 1(Institute of the Functional Molecule,Department of Chemistry,South China University of Technology,Guangzhou 510641) 2(Guangzhou City Polytechnic,Guangzhou 510405) 3(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405);Effect of Fatty Acids Methyl Ester from Plastrum Testudinis on Proliferation of Rat Bone Mesenchymal Stem Cell[J];Chinese Journal of Analytical Chemistry;2007-10
3 Li Yiwei,Zhou Jianhong,Chen Dongfen,Li Hui,Du Shaohui.Guangzhou University of TCM,Guangzhou 510405 China;Effect of Tortoises shell on behavior and striatum levels of dopamine in rat model of Parkinsons disease[J];Anatomy Research;2004-01
4 HU Xiang;LU Hua;Clinical Medicine College of Chengdu University of Traditional Chinese Medicine;The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine;;Effects of rat medicated sera prepared with Yougui Wan Formulae on differentiation of human FTM-PVCs into oocyte-like cells in vitro[J];China Journal of Traditional Chinese Medicine and Pharmacy;2014-05
5 DU Pei-lin;ZHOU Yu-qing;ZHU Hua;Guangxi University of Chinese Medicine;;Research Progress of the Carapax et Plustrum Testudinis[J];Journal of Anhui Agricultural Sciences;2014-32
6 CHAI Li-juan,WANG Shao-xia,GUO Hong,ZHUANG Peng-wei,GAO Xiu-mei(Pharmaceutical college of Tianjing University of Chinese Medicine,Tianjing 300193,China);Differentiation regulation of neural stem cell and the affection of the Chinese traditional medicine on it[J];Strait Pharmaceutical Journal;2008-10
7 WANG Chun-yan,ZENG He-ping,CHEN Wei(School of Chemistry & Environment,South China Normal University Guangzhou 510631,China);EFFECTS ON PROLIFERATION OF RAT MESENCHYMAL STEM CELL AND CHEMICAL COMPONENTS FROM PLASTRUM TESTUDINIS OBTAINED BY GC-MS[J];Journal of South China Normal University(Natural Science Edition);2007-01
8 ao Jianfeng,Li Jiansheng Geroatrics Research Laboratory, Henan College of Traditional Chinese Medicine;The Present Situation in Studies on Interfering Inflammatory Cascade Peaction Caused by Cerebral Ischemia[J];Journal of Henan College of Traditional Chinese Medicine;2005-02
9 XUAN Ya-bo,LIU Hong(Department of Acupuncture and Moxibustion,Beijing Hospital of Chinese Medicine,Capital University of Medical Sciences,Beijing 100010);Influence of Peiyuantongnao Capsules on blood fat in patients of stroke convalescence[J];Journal of Beijing University of Traditional Chinese Medicine(Clinical Medicine);2011-01
10 WANG Li-xin,CAI Ye-feng,SUN Jing-bo(Center of Cerebral Vessel Diseases,Guangdong Provincial TCM Hospital,Guangzhou,Guangdong,510120,China);TCM Insight of Proliferation and Differentiation of Endogenous Neural Stem Cells after Cerebral Ischemia[J];Journal of Nanjing University of Traditional Chinese Medicine;2008-04
China Proceedings of conference Full-text Database 1 Hits
1 HU Xiang;LU Hua;Clinical Medicine College of Chengdu University of Traditional Chinese Medicine;The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine;;Effects of rat medicated sera prepared with Yougui Wan Formulae on differentiation of human FTM-PVCs into oocyte-like cells in vitro[A];[C];2014
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhou Yixuan,Dai Xiaohua,Li Chonghui,Wang Ling, Zhang Nianzhi,Gu Jianxia,Liang Xinglun (The First Affiliated Hospital,Anhui College of TCM,Hefei 230031);42 CASES OF ISCHEMIC HEART FAILURE TREATED BY GINSENG INJECTION[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;1999-05
2 LI Ping,ZHANG Mei (Dept.of TCM,Anhui Provincial Hospital,Anhui Hefei 230001,China);EFFECTS OF ASTRAGALUS ON APOPTOSIS RELATED GENE EXPRESSION IN RATS AFTER CEREBRAL ISCHEMIA[J];Journal of Anhui Traditional Chinese Medical College;2001-04
3 GAO Hui,LI Xian-hui,LI Chun-yan,L Jiang-ming(Department of Pharmacology,Medical College of Jishou University,Jishou 416000,China);Effect of salvianolate on the platelet aggregation and adhesiveness in healthy volunteers[J];Journal of Bengbu Medical College;2007-05
4 Yu Qin,Lian Junlan,Wang Yan,et al Zhejiang University of Traditional Chinese Medicine,Hangzhou(310053);Study on Method of Expanding and Culturing Rat Mesenchymal Stem Cells in vitro and Its Biological Characteristics[J];Journal of Zhejiang University of Traditional Chinese Medicine;2006-02
5 Wang Xukai1, Wang Ying2,Yang Yougeng, et al 1 The Second Hospital of Jilin University, Changchun(130041), China;2 The Second Affiliated Hospital of Tianjin University of TCM;Effects of the Velvet Antler Polypeptides on Spinal Nerve Cell Apoptosis Induced by β-amyloid Peptide[J];Journal of Zhejiang University of Traditional Chinese Medicine;2009-01
6 Lv Guiyuan1,Chen Suhong2,Su Jie1,et al 1.Zhejiang Chinese Medical University,Hangzhou(310053);2.Wenzhou Medical College;Effects of Different Fractions from Cortex Eucommiae on Oxidation Resistance and Anti-despondent of Extradiol-induced Kidney-yang Deficiency Mice[J];Journal of Zhejiang University of Traditional Chinese Medicine;2009-05
7 CHANG Yan, XUE Yi-long, LUO Yun, et al. (Cytology Lab., Institute of Geriatrics, PLA General Hospital, Beijing 100853, China);The Cholinergic-inducing Effect of BMP4 on Rat′s Cerebral Neural Stem Cells[J];Labeled Immunoassays and Clinical Medicine;2004-01
8 Li Ying 1,Cui Xinming 1,Pan Li 1,Li Zhenbao 1,Ji Chongde 2,Du Kuiqin 3 (1\ Dept.of Electron Microscopy;2\ Dept.of General Surgery,The Third Clinical College;3\ Dept.of Pathophysiology);Electron microscopic observation and Ca~(2+) analysis on protective effect of ginsenoside from fruit on myocardium of hemorrhagic shock in dogs[J];Journal of Norman Bethune University of Medical Science;1998-05
9 WANG Ke-li~1,LU Lai-jin~(1),GONG Xu~1,LI Xiang-jun~2(1.Department of Hand Surgery,First Hospital,Jilin University,Changchun,130021,China;2.Department of Pathology,School of Pharmacy,Jilin University,Changchun 130021,China);Enhancement effects of velvet antler polypeptides-PLGA compound membrane on peripheral nerve regeneration in rats[J];Journal of Jilin University(Medicine Edition);2006-02
10 NIU Yun1,HE Xu1,WANG Xin-rui2,MA Ying-zhi1,LI Yu-lin1(1.Key Laboratory of Pathobiology,Ministry of Education School of Basic Medical Sciences,Jilin University,Changchun 130021,China; 2.Key Laboratory of Zoonosis,Ministry of Education, Institute of Zoonosis,Jilin University,Changchun 130062,China);Differentiation of bone mesenchymal stem cells into neuron-like cells induced by supernatant of glioma cells[J];Journal of Jilin University(Medicine Edition);2007-04
China Proceedings of conference Full-text Database 1 Hits
1 Zhang jin,Huang jin,Xu Zhi-wei Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Effect of invigorating kidney and nourishing essence on MSCs proliferation in vitro and its mechanism[A];[C];2010
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ;龟甲"滋阴补肾"药效相关研究概况[J];Contemporary Medicine;2009-10
2 JIANG Ze-dong,LI Xia,QIN Yan-jie,GE Shan-shan(Key Laboratory of Marine Bio-resources Sustainable Utilization in Liaoning Provinc's University,Dalian Fisheries Univ.,Dalian 116023,China);The effects of growth factors and hormones on growth of the cultured cells in turbot Scophthalmus maximus in vitro[J];Journal of Dalian Fisheries University;2009-05
3 DU Hong-yang,FU Hai-yan,MA Gang,BAO Cui-fen,QIN Shu-jian.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;Effect of rehmannia glutinosa polysaccharide on the expression of Notch1 protein in rat bone marrow mesenchymal stem cells during differentiation into neuron-like cells in vitro[J];Guangdong Medical Journal;2012-09
4 CAI Xue-yan,XIE Hua,CUI Xiao-jun, HUANG Zhi-wei, NA Qing-qing, SU Kai-xin, LIN Yan-sheng Department of Anatomy, Guangdong Medical College, Guangdong 524023 China;Function of Buyang-Huanwu decoction on transdifferentiation of mesenchymal stem cells transplantation after spinal cord injury in rats[J];Anatomy Research;2008-03
5 LI Chun, CHEN Lan, LI Hui, DENG Ru-dong, ZHANG Sai-xia, CHEN Dong-feng, Li Yi-wei.Department of Anatomy, the School of Preclinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Plastrum Testudinis Extact Inhibited Rat Fetal Epidermal Stem Cells Apoptosis Indued By Ultra-Violet Radiation[J];Anatomy Research;2010-03
6 WU Qing-guang1,LIANG You-ya1,LIU Si-jun2,JIANG Qian-e1(1.School of Chinese Herbal Medicine;2.Institute of Clinical Pharmacology,Guangzhou University of Chinese Medicine,Guangzhou 510405,China);Research progress in testacean of traditional Chinese medicine[J];Journal of Guangdong Pharmaceutical University;2012-02
7 XIE Xing-wen,LI Jing,XU Wei Gansu Province Academy of TCM,Lanzhou 730050,China;Function of Aqueous Extract from QiangHuoYu on Formation of Rats' Bone Marrow Mesenchymal Stem Cells[J];Western Journal of Traditional Chinese Medicine;2012-01
8 WANG Bin,LUO Yi-wen,HU Nian-hong(Affiliated Orthopaedic Hospital of Guangzhou University of Traditional Chinese Medicine,Guangdong Guangzhou 510240,China);Effects of Traditional Chinese Medicine Formula for Nourishing Kidney-Essence on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Rabbits in Different Phases of Fracture Healing[J];Journal of Anhui Traditional Chinese Medical College;2013-01
9 ZHANG Hai-ling;CHEN jin-feng;CHEN dong-feng;ZHENG Er-lai;YE mao-sheng;HUA Zi-chun;Department of Anatomy,Zhaoqing Medicine College;;Extracts from Plastrum Testudinis regulates the expression of Id1 in BMSCs[J];Anatomy Research;2013-05
10 WAN Qian;LU Hua;DENG Yanli;LIANG Limei;Reproduction Laboratory of Traditional Chinese Medicine and Western Medicine, Second Affiliated Clinical Medical School, Chengdu University of Traditional Chinese Medicine;Sichuan Family Planning Research Institute, Chengdu University of Traditional Chinese Medicine;Second Affiliated Hospital, Chengdu University of Traditional Chinese Medicine;Affiliated Clinical Medical School, Chengdu University of Traditional Chinese Medicine;;Psoralen induces rat mesenchymal stem cell differentiation towards male germ cells in vitro[J];Journal of Southern Medical University;2013-12
China Proceedings of conference Full-text Database 1 Hits
1 Zhangchen,Huangjin,Zhangjin,Zhanfei (School of Basic Medical Sciences,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China);Based on stem cells "kidney essence" theory and mechanism of aging[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 CHEN Dongfeng 1, DU Shaohui 2, LI Yiwei 1, ZHANG Jin 1, LI Hui 1 (1.Dept.of Anatomy,Guangzhou University of TCM,Guangzhou 510405,China; 2.Shenzhen Hospital of TCM,Shenzhen 518033,China);Effect of Plastrum Testudinis on Neural Stem Cell After Focal Cerebral Ischemia[J];Journal of Guangzhou University of Traditional Chinese Medicine;2001-04
2 BIANWEI, YANGJING, TANGKE, JINGNAI HE  (Shanghai Institute of Biochemistry, Chinese Academy of Science; Shanghai Research Center of Life Sciences, Shanghai 200031);NESTIN EXPRESSION DURING P19 NEURON DIFFERNTIATION *[J];ACTA PHYSIOLOGICA SINICA;1999-03
3 DENG Qiang; XU Hong; ZHU Dong-Ya;(New Drug Research Center, China Pharmaceutical University, Nanjing 210009);Relationship between iNOS and the late-phase cerebral ischemia[J];CHINESE BULLETIN OF LIFE SCIENCES;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved