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《Journal of Guangzhou University of Traditional Chinese Medicine》 2005-04
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Construction of Subtractive cDNA Library from the Leaves of Dendrobium candidum Wall, ex Lindl. by Suppression Subtractive Hybridization Technology

WEI Xiaoyong (Dept. of Histology and Embryology, Guangzhou University of TCM, Guangzhou 510405)  
[Objective] Subtractive cDNA libraries from the leaves of D. candidum were constructed to supply evidence for the clone of dendrobine-biosynthesis-associated genes. [ Methods ] Suppression subtractive hybridization ( SSH) technology was used. With the cDNA of perennial leaf as the tester and that of annual leaf as the driver, forward and reverse hybridization was performed. The two obtained subtracted cDNA fragments were cloned into PointTMXa-1 plasmid vectors, and then the vectors were transformed into E. Coli JM109. The inserted fragments were amplified by PCR and then identified. [Results] The subtractive cDNA libraries related with dendrobine-biosynthesis-associated genes were successfully constructed. The forward and reverse subtractive libraries included 560 and 220 clones respectively. Detected by PCR, the length of the inserted fragments was 550 bp on average. [Conclusion] The constructed subtractive libraries are suitable for further study on the functional genes associated with dendrobine biosynthesis of D. candidum.
【Fund】: 广东省中医药管理局资助课题(编号:402003 303005)
【CateGory Index】: R282.5
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【References】
Chinese Journal Full-text Database 1 Hits
1 Huang Xin1,2 Dai Silan1 Meng Li1,2 Zheng Guosheng2* 1 College of Landscape Architecture, Beijing Forestry University, Beijing, 100083; 2 College of Life Science, Laiyang Agriculture College, Qingdao, 266109;The Application of Suppression Subtractive Hybridization (SSH) on Isolating Plant Different Genes[J];Molecular Plant Breeding;2006-05
【Co-references】
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1 OUYANG Jian-Hua; FANG Feng; WANG Jun-Ying;SHEN Qian (1. Department of Pediatrics, Changhai Hospital, Second Military Medical University,Shanghai 200433,China; 2. Department of Laboratory Diagnosis);Changes of T lymphocyte subpopulation,NK and LAK cell activities in multiviral infection[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;2000-04
2 ZHANG You lin,CHEN Jin ping,Su Dong hua (Department of Food Engineering,Shaanxi Normal University,Xian 710062,China);Studies on the change of ABA content and respiratory non-climactic character after harvest in grape and fresh Jujube[J];Acta Botanica Boreali-occidentalia Sinica;2002-05
3 REN Xiao-lin~1,LI Hai-feng~2, GONG De-qiang~1, ZHANG Shao-ying~1(1 College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100,China; 2 Jinta Company, Department of Landscape Architecture of Han County, Han County, Shaanxi 715401,China);Effects of application of ethephon and GA_3 in fall on bud sprouting and flowering of peony[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
4 LIU Bo~1,ZHENG Guo-sheng~(2*),YAN Zhi-pei~3,WANG Zong-zheng~1 (1 College of Life Science,Shandong Agricultural University,Tai'an,Shandong 271018,China;2 College of Life Science,Laiyang Agricultural College(Qingdao Branch Campus),Qingdao 266109,China;3 Department of Biology,Zaozhuang Teacher's College,Zaozhuang,Shandong 277160,China);Effect of different chilling treatments on forcing culture of tree peony in winter and changes of nutrient substance[J];Acta Botanica Boreali-occidentalia Sinica;2004-09
5 CHEN Fan,HE Qi gai,CHEN Huan chun,LIU Zhen fei (Veterinary Medicine College,Huazhong Agricultural University,Wuhan,Hubei,430070,China);The Advance on Serotyping of Actinobacilus pleuropneumoniae[J];Progress In Veterinary Medicine;2004-01
6 XIE Bao-dong,HE Hou-jun,ZHU Ren-fa (Animal Science and!Technology College, Jiangxi Agriculture University,Nanchang,Jiangxi,330045,China);Advance in Virulence Factors of Porcine Actinobacillus pleuropneumonia[J];Progress In Veterinary Medicine;2005-05
7 ZHAO Hui-fang~(1,2 ), DING De-rong~(1), MA Zhong-hua~(1), KUAI Ben-ke~(1) (1.Department of Biochemistry, School of Life Sciences, Fudan University,Shanghai 200433, China; 2. Department of Biology, School of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China);Isolation of Genes with Enhanced Expressions in Rice Treated with Probenazole Using SSH Technique[J];Journal of Fudan University;2004-02
8 SONG Gang, LIN Shun quan, LU Liu xin, MA Ying (Department of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Advances in applying antisense RNA technology to controlling fruit maturation[J];Journal of Fujian Agricultural University(Natural Science);2000-04
9 Xu Biyu Su Wei Jin Zhiqiang*State Key Laboratory of Tropical Crops Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101;Construction of Differentially Expressed cDNA Library of Banana Fruit by Using Suppression Subtractive Hybridization[J];Molecular Plant Breeding;2005-04
10 Huang Xin1,2 Dai Silan1 Meng Li1,2 Zheng Guosheng2* 1 College of Landscape Architecture, Beijing Forestry University, Beijing, 100083; 2 College of Life Science, Laiyang Agriculture College, Qingdao, 266109;The Application of Suppression Subtractive Hybridization (SSH) on Isolating Plant Different Genes[J];Molecular Plant Breeding;2006-05
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 Xie Linan Nie Yuzhe Zhang Xiaolei Li Yuhua Department of Life Science, Northeast Forestry University, Harbin, 150040;Construction and Analysis of Suppression Subtractive Hybridization Library of Leymus chinensis under Saline-alkaline Stress[J];Molecular Plant Breeding;2007-03
2 ZHAO Hu (1.Department of Biology,Fuyang Teachers College,Fuyang,236041;2.Biology Technology Institute Chinese Academy of Agricural Sciences,Beijing,China 100081);Construction of cDNA library of rice seedlings roots under nap stress based on suppression subtractive hybridization[J];Journal of Biology;2008-03
3 LIU Guan-Jun, LIU Ming-Kun, XU Zhi-Ru, YAN Xiu-Feng, WEI Zhi-Gang, YANG Chuan-Ping The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China;Construction and analysis of a forward and reverse subtractive cDNA library from leaves and stem of Polygonum sibiricum Laxm.under salt stress[J];Hereditas;2009-04
4 HUANG Min1,WANG Yan1,PAN Lin1,YANG Cheng-ming1,SUN Qun2,GAO Peng1,WU Ling1,DU Xiao-ying1,LEI Qing 1 (1.Sichuan Institute of Atomic Energy,Sichuan Chendu 610066,China;2.College of Life Sciences,Sichuan University,Sichuan Chengdu 610064,China);Screening and Identification of Heat Tolerance Genes of Hybrid Rice[J];Southwest China Journal of Agricultural Sciences;2009-05
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